Pathway-Specific Trafficking of Native AMPARs by In Vivo Experience

An accumulating body of evidence supports the notion that trafficking of AMPA receptors (AMPARs) underlies strengthening of glutamatergic synapses and, in turn, learning and memory in the behaving animal. However, without exception, these experiments have been performed using artificial stimulation...

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Bibliographic Details
Published in:Neuron (Cambridge, Mass.) Mass.), 2006-03, Vol.49 (5), p.663-670
Main Authors: Clem, Roger L., Barth, Alison
Format: Article
Language:English
Subjects:
Analysis of Variance
Animals
Animals, Newborn
Araneae
Calcium - metabolism
Dose-Response Relationship, Radiation
Electric Stimulation - methods
Excitatory Postsynaptic Potentials - drug effects
Excitatory Postsynaptic Potentials - physiology
Excitatory Postsynaptic Potentials - radiation effects
Experiments
Green Fluorescent Proteins - genetics
In Vitro Techniques
Mice
Mice, Transgenic
MOLNEURO
Neocortex - cytology
Neural Pathways - physiology
Neurons
Neurons - physiology
Oncogene Proteins v-fos - metabolism
Patch-Clamp Techniques - methods
Protein Transport - physiology
Ratios
Receptors, AMPA - metabolism
Rodents
Synapses - drug effects
Synapses - physiology
Synapses - radiation effects
SYSNEURO
Transgenic animals
Vibrissae - innervation
Vibrissae - physiology
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Summary:An accumulating body of evidence supports the notion that trafficking of AMPA receptors (AMPARs) underlies strengthening of glutamatergic synapses and, in turn, learning and memory in the behaving animal. However, without exception, these experiments have been performed using artificial stimulation protocols, cultured neurons, or viral-overexpression systems that can significantly alter the normal function of AMPARs. Using a single-whisker experience protocol that significantly enhances neuronal responses in vivo, we have targeted neurons in and around the spared whisker column of fosGFP transgenic mice for whole-cell recording. Here we show that in vivo experience induces the pathway-specific strengthening of neocortical excitatory synapses. By assaying AMPARs for rectification and sensitivity to joro spider toxin, we find that in vivo experience induces the delivery of native GluR2-lacking receptors at spared, but not deprived, inputs. These data demonstrate that pathway-specific trafficking of GluR2-lacking AMPARs is a normal feature of synaptic strengthening that underlies experience-dependent plasticity in the behaving animal.
ISSN:0896-6273
1097-4199
DOI:10.1016/j.neuron.2006.01.019