Monitoring of Fusarium species in soybean root by DNA array hybridization

Accurate identification of Fusarium species currently presents a challenge to plant pathologists and mycologists. A representative set of 219 Fusarium isolates was selected from a total of 876 isolates recovered from soybean roots during the spring seasons of 2001 and 2002 from 116 commercial fields...

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Bibliographic Details
Published in:Phytopathology 2005-06, Vol.95 (6)
Main Authors: Levesque, C, Barasubiye, T, Seifert, K, Tenuta, A, Rioux, S, Anderson, T, Welacky, T
Format: Article
Language:English
Subjects:
Fusarium
Fusarium proliferatum
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Summary:Accurate identification of Fusarium species currently presents a challenge to plant pathologists and mycologists. A representative set of 219 Fusarium isolates was selected from a total of 876 isolates recovered from soybean roots during the spring seasons of 2001 and 2002 from 116 commercial fields in eastern Ontario and Quebec, Canada. Following partial DNA sequencing of the EF1-alpha gene, eleven groups were found, namely, F. oxysporum complex, F. solani complex, F. graminearum complex, F. sporotrichioides, F. tricinctum, F. equiseti complex, F. proliferatum, F. sambucinum, F. acuminatum and Fusarium sp cf merismoides. Based on our sequences and other databases (i.e. GenBank, and unpublished sequences received from K. O'Donnell), we designed 71 species or clade specific oligonucleotides to target the main species of Fusarium isolated in this study. This novel EF1-alpha DNA array consistently identified and detected Fusarium species directly in soybean roots, including the Fusarium species implicated in soybean sudden death syndrome disease in North America.
ISSN:0031-949X