Targeted Silencing of Kim-1 Inhibits the Growth of Clear Cell Renal Cell Carcinoma Cell Line 786-0 In Vitro and In Vivo

• Published in: Oncology research 2018-08, Vol.26 (7), p.997-1003
• Main Authors: Xu, Jianping, Sun, Liguo, Sun, Wei, Tian, Jianhai, Guo, Huaiyuan
• Format: Article
• Language: English
• Subjects: Animals; Apoptosis; Biomarkers, Tumor - genetics; Biomarkers, Tumor - metabolism; Carcinoma, Renal Cell - genetics; Carcinoma, Renal Cell - metabolism; Carcinoma, Renal Cell - pathology; Cell Cycle; Cell Proliferation; Clear Cell Renal Cell Carcinoma; Down-Regulation; Female; Hepatitis A Virus Cellular Receptor 1 - antagonists & inhibitors; Hepatitis A Virus Cellular Receptor 1 - genetics; Hepatitis A Virus Cellular Receptor 1 - metabolism; Humans; In Vitro Techniques; Kidney Injury Molecule-1 (kim-1); Kidney Neoplasms - genetics; Kidney Neoplasms - metabolism; Kidney Neoplasms - pathology; Lentivirus; Mice; Mice, Inbred BALB C; Mice, Nude; Oxaliplatin; Proliferation; Rna Interference; RNA, Small Interfering - genetics; Tumor Cells, Cultured; Xenograft Model Antitumor Assays
• ISSN: 0965-0407; 1555-3906
• DOI: 10.3727/096504017X15140544654946

To investigate the effect of Kim-1 on 786-0 cells in vivo and in vitro, several experiments such as quantitative real-time PCR, Western blot, MTT, colony formation, and flow cytometry were performed to evaluate the biological behavior of 786-0 cells treated with Kim-1 siRNA. Furthermore, the tumor xenograft model was applied to BALB/c nude mice to assess the effect of Kim-1 silencing. Lentivirus-mediated RNAi effectively silenced Kim-1 in 786-0 cells. Kim-1 knockdown significantly inhibited the proliferation and colony formation ability of 786-0 cells (p