Analysis of circulating extracellular vesicle-associated microRNAs in cortisol-producing adrenocortical tumors

Purpose Circulating microRNAs (miRNA) have been described in patients with adrenocortical tumors, but the expression of miRNAs in non-functioning and cortisol-producing tumors has not been yet compared. Therefore, the objective of this study was to evaluate the expression of plasma extracellular ves...

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Bibliographic Details
Published in:Endocrine 2018-02, Vol.59 (2), p.280-287
Main Authors: Perge, Pál, Decmann, Ábel, Pezzani, Raffaele, Bancos, Irina, Fassina, Ambrogio, Luconi, Michaela, Canu, Letizia, Tóth, Miklós, Boscaro, Marco, Patócs, Attila, Igaz, Peter
Format: Article
Language:English
Subjects:
Adenoma
Adrenal Cortex Neoplasms - genetics
Adrenal Cortex Neoplasms - metabolism
Adrenocortical Adenoma - genetics
Adrenocortical Adenoma - metabolism
Adrenocortical Carcinoma - genetics
Adrenocortical Carcinoma - metabolism
Adult
Aged
Aged, 80 and over
Circulating MicroRNA - genetics
Circulating MicroRNA - metabolism
Cortisol
Dexamethasone
Diabetes
Endocrine Genetics/Epigenetics
Endocrinology
Extracellular Vesicles - metabolism
Female
Hormones
Humanities and Social Sciences
Humans
Hydrocortisone - metabolism
Internal Medicine
Male
Medicine
Medicine & Public Health
MicroRNAs
Middle Aged
miRNA
multidisciplinary
Science
Tumors
Young Adult
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Summary:Purpose Circulating microRNAs (miRNA) have been described in patients with adrenocortical tumors, but the expression of miRNAs in non-functioning and cortisol-producing tumors has not been yet compared. Therefore, the objective of this study was to evaluate the expression of plasma extracellular vesicle (EV)-associated microRNAs in patients with non-functioning adrenocortical adenoma (NFA), cortisol-producing adrenocortical adenoma (CPA) and cortisol-producing adrenocortical carcinoma (CP-ACC). Methods Preoperative plasma EV samples of 13 NFAs, 13 CPAs and 9 CP-ACCs were subjected to extracellular vesicle isolation. miRNAs were investigated by targeted quantitative real-time PCR normalized to cel-miR-39 as reference. Five miRNAs have been selected for this analysis based on the previous studies including hsa-miR-22-3p, hsa-miR-27a-3p, hsa-miR-210-3p, hsa-miR-320b and hsa-miR-375. Results We have observed significant overrepresentation of three miRNAs in both CPA and CP-ACC relative to NFA: hsa-miR-22-3p ( p  
ISSN:1355-008X
1559-0100
DOI:10.1007/s12020-017-1506-z