Fibroblast growth factor receptor 1 and 3 expression is associated with regulatory PI3K/AKT kinase activity, as well as invasion and prognosis, in human laryngeal cancer

• Published in: Cellular oncology (Dordrecht) 2018-06, Vol.41 (3), p.253-268
• Main Authors: Starska, Katarzyna, Forma, Ewa, Lewy-Trenda, Iwona, Stasikowska-Kanicka, Olga, Skóra, Michał, Bryś, Magdalena
• Format: Article
• Language: English
• Subjects: 1-Phosphatidylinositol 3-kinase; Aged; Aged, 80 and over; AKT protein; Biomarkers; Biomarkers, Tumor - metabolism; Biomedical and Life Sciences; Biomedicine; Cancer Research; Cohort Studies; Female; Fibroblast growth factor receptor 1; Fibroblast growth factor receptors; Fibroblasts; Gene expression; Growth factors; Head & neck cancer; Humans; Immunohistochemistry; Invasiveness; Kaplan-Meier Estimate; Kinases; Laryngeal cancer; Laryngeal Mucosa - metabolism; Laryngeal Mucosa - pathology; Laryngeal Neoplasms - diagnosis; Laryngeal Neoplasms - metabolism; Laryngeal Neoplasms - mortality; Laryngeal Neoplasms - pathology; Male; Medical prognosis; Middle Aged; Mucosa; Neoplasm Grading; Neoplasm Invasiveness; Neoplasm Recurrence, Local; Neoplasms, Squamous Cell - diagnosis; Neoplasms, Squamous Cell - metabolism; Neoplasms, Squamous Cell - mortality; Neoplasms, Squamous Cell - pathology; Oncology; Original Paper; Pathology; Phosphatidylinositol 3-Kinases - genetics; Phosphatidylinositol 3-Kinases - metabolism; Prognosis; Protein expression; Proteins; Proto-Oncogene Proteins c-akt - genetics; Proto-Oncogene Proteins c-akt - metabolism; Receptor, Fibroblast Growth Factor, Type 1 - genetics; Receptor, Fibroblast Growth Factor, Type 1 - metabolism; Receptor, Fibroblast Growth Factor, Type 3 - genetics; Receptor, Fibroblast Growth Factor, Type 3 - metabolism; Snail Family Transcription Factors - metabolism; Western blotting
• ISSN: 2211-3428; 2211-3436
• DOI: 10.1007/s13402-017-0367-z

Purpose Aberrant fibroblast growth factor receptor (FGFR) expression is thought to contribute to the development of many types of cancer. As yet, however, their impact on the course and prognosis of head and neck cancer remains to be determined. Here, we aimed to investigate the effects of expression of the FGFR family members FGFR1 and FGFR3, as well as their downstream PI3K/AKT signal-regulated kinases, on the aggressiveness and prognosis of laryngeal cancer. Methods In total 137 surgically removed squamous cell laryngeal cancer (SCLC) and 100 matched non-cancerous laryngeal mucosa (NCLM) samples were assessed for mRNA expression using quantitative real-time PCR. The corresponding proteins were analyzed by Western blotting. SLUG expression was assessed by immunohistochemistry. The expression data were subsequently related to tumor front grading (TFG), local/nodal recurrences, prognosis and overall survival. Results The FGFR1, FGFR3 and PI3K/AKT kinase mRNA and protein levels were found to be significantly higher in the SCLC than the NCLM samples ( p