Identification and characterisation of thirteen new microsatellites for Atlantic cod (Gadus morhua L.) from a repeat-enriched library

A total of 13 polymorphic microsatellite markers were developed for Atlantic cod (Gadus morhua L.) from a repeat-enriched library. Polymorphism of each locus was assessed in 96 unrelated individuals from a natural population. The number of alleles per locus varied from 8 to 45. The ranges of observe...

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Bibliographic Details
Published in:Conservation genetics 2007-06, Vol.8 (3), p.749-751
Main Authors: Westgaard, Jon-Ivar, Tafese, Tekle, Wesmajervi, Mette Serine, Stenvik, Jørgen, Fjalestad, Kjersti Turid, Damsgård, Børge, Delghandi, Madjid
Format: Article
Language:English
Subjects:
Enriched genomic library
Fish
Gadus morhua
Genetic markers
Genetically modified organisms
Genetics
Heterozygosity
Libraries
Linkage disequilibrium
Loci
Marine
Markers
microsatellite repeats
Microsatellites
Polymorphism
Population genetics
Population studies
Reproduction
Type II markers
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Summary:A total of 13 polymorphic microsatellite markers were developed for Atlantic cod (Gadus morhua L.) from a repeat-enriched library. Polymorphism of each locus was assessed in 96 unrelated individuals from a natural population. The number of alleles per locus varied from 8 to 45. The ranges of observed and expected heterozygosity were 0.122-0.907 and 0.673-0.965, respectively. Four of the loci (Gmo-G24, Gmo-G40, Gmo-G46 and Gmo-G49) followed Hardy-Weinberg expectation. No evidence for linkage disequilibrium between pairs of loci was found in any combination of loci pairs, except between Gmo-G40 and Gmo-G43. These microsatellite markers provide useful tools for studies of population genetics, reproductive ecology and for constructing linkage maps of Atlantic cod.
ISSN:1566-0621
1572-9737
DOI:10.1007/s10592-006-9200-3