Growth characteristics of virulent Bacillus anthracis and potential surrogate strains

The objectives of this study were to compare generation and lag times of virulent Bacillus anthracis strains with those of other Bacillus strains, to identify possible surrogates for growth studies, and to determine if the B. cereus module of the U.S. Department of Agriculture Pathogen Modeling Prog...

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Bibliographic Details
Published in:Journal of food protection 2006-07, Vol.69 (7), p.1720-1723
Main Authors: De Siano, T, Padhi, S, Schaffner, D.W, Montville, T.J
Format: Article
Language:English
Subjects:
airborne microorganisms
anthrax
Bacillus - growth & development
Bacillus - pathogenicity
Bacillus anthracis
Bacillus anthracis - growth & development
Bacillus anthracis - pathogenicity
Bacillus cereus
Bacillus cereus - growth & development
Bacillus cereus - pathogenicity
Bacillus mycoides
Bacillus subtilis
Biological and medical sciences
Bioterrorism
Colony Count, Microbial
Consumer Product Safety
culture media
Food Contamination - analysis
Food industries
Food Microbiology
food pathogens
Fundamental and applied biological sciences. Psychology
Humans
Hydrogen-Ion Concentration
Kinetics
microbial growth
Models, Biological
predictive microbiology
Predictive Value of Tests
serotypes
spore-forming bacteria
viability
Virulence
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Summary:The objectives of this study were to compare generation and lag times of virulent Bacillus anthracis strains with those of other Bacillus strains, to identify possible surrogates for growth studies, and to determine if the B. cereus module of the U.S. Department of Agriculture Pathogen Modeling Program (PMP) had predictive value for B. anthracis. Growth characteristics of B. anthracis, B. cereus, B. mycoides, and B. subtilis strains in brain heart infusion broth at pH 6.5, 6.0, and 5.5 were determined by absorbance measurements. Growth curves of B. anthracis Sterne and B. cereus strains appeared similar, and the generation times for strain Sterne fell within the PMP's 95% confidence interval for B. cereus. However, the virulent B. anthracis strains Vollum and Pasteur had shorter generation times than the avirulent Sterne strain and most other surrogates and were lower than the PMP's 95% confidence interval for B. cereus. Growth curves of B. cereus ATCC 9818 and B. subtilis ATCC 6633 were more similar to those of virulent B. anthracis strains, but all potential surrogates had significantly different generation times and lag times under some conditions.
ISSN:0362-028X
1944-9097
DOI:10.4315/0362-028X-69.7.1720