Post-harvest enhancement of aroma in transgenic lisianthus ( Eustoma grandiflorum) using the Clarkia breweri benzyl alcohol acetyltransferase ( BEAT) gene

Lisianthus ( Eustoma grandiflorum) is an ornamental plant with beautiful but scentless flowers. In an attempt to induce a fragrance in their flowers, lisianthus plants were transformed with the Clarkia breweri gene coding for benzyl alcohol acetyltransferase ( BEAT), catalyzing the synthesis of the...

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Bibliographic Details
Published in:Postharvest biology and technology 2007-02, Vol.43 (2), p.255-260
Main Authors: Aranovich, Dina, Lewinsohn, Efraim, Zaccai, Michele
Format: Article
Language:English
Subjects:
benzyl alcohol acetyltransferase
Biological and medical sciences
chemical constituents of plants
Clarkia
Clarkia breweri
Eustoma grandiflorum
Eustoma russellianum
flowers
Food industries
Fragrance
Fruit and vegetable industries
Fundamental and applied biological sciences. Psychology
genes
genetic engineering
headspace analysis
Lisianthus
odors
plant genetics
postharvest treatment
Transformation
transgenic plants
volatile compounds
Volatiles
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Summary:Lisianthus ( Eustoma grandiflorum) is an ornamental plant with beautiful but scentless flowers. In an attempt to induce a fragrance in their flowers, lisianthus plants were transformed with the Clarkia breweri gene coding for benzyl alcohol acetyltransferase ( BEAT), catalyzing the synthesis of the volatile compound benzyl acetate under the regulation of the CaMV35S promoter. An external supply of benzyl alcohol induced five to seven times higher production of benzyl acetate in detached flowers and leaves of transgenic lisianthus plants, compared to non-transformed plants. No benzyl acetate was detected in tissues of both control and transgenic plants fed with water. When fed with additional alcoholic compounds, i.e. hexanol, benzyl alcohol, isoamyl alcohol, phenethyl alcohol, and cinnamyl alcohol, assumed to be used as substrates by BEAT, transgenic in vitro-grown lisianthus plantlets produced significantly higher levels of acetates than control plants. These results demonstrate the possibility of producing substrate-dependent acetates in transgenic lisianthus plants, which could lead to induction of new aromas.
ISSN:0925-5214
1873-2356
DOI:10.1016/j.postharvbio.2006.09.001