Comparison of ovine herpesvirus 2 genomes isolated from domestic sheep (Ovis aries) and a clinically affected cow (Bos bovis)

The rhadinovirus Ovine herpesvirus 2 (OvHV-2) is the causative agent of sheep-associated malignant catarrhal fever. OvHV-2 primarily affects ruminants and has a worldwide distribution. In this study, a composite sequence of OvHV-2 genomic DNA isolated from nasal secretions of sheep experiencing viru...

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Bibliographic Details
Published in:Journal of general virology 2007, Vol.88 (1), p.40-45
Main Authors: Taus, N.S, Herndon, D.R, Traul, D.L, Stewart, J.P, Ackermann, M, Li, H, Knowles, D.P, Lewis, G.S, Brayton, K.A
Format: Article
Language:English
Subjects:
Amino Acid Sequence
amino acid sequences
Animals
Base Sequence
Biological and medical sciences
Bos
Cattle
Cattle Diseases - virology
DNA, Viral - analysis
DNA, Viral - blood
Fundamental and applied biological sciences. Psychology
genetic polymorphism
genome
Herpesviridae Infections - veterinary
Herpesviridae Infections - virology
isolation
malignant catarrhal fever
Microbiology
Miscellaneous
Molecular Sequence Data
Nasal Mucosa - virology
nucleotide sequences
open reading frames
Ovine herpesvirus 2
Ovis aries
Rhadinovirus
Rhadinovirus - chemistry
Rhadinovirus - genetics
Rhadinovirus - isolation & purification
Ruminantia
Sheep
Sheep Diseases - virology
strain differences
strains
Virology
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Summary:The rhadinovirus Ovine herpesvirus 2 (OvHV-2) is the causative agent of sheep-associated malignant catarrhal fever. OvHV-2 primarily affects ruminants and has a worldwide distribution. In this study, a composite sequence of OvHV-2 genomic DNA isolated from nasal secretions of sheep experiencing virus-shedding episodes was determined and compared with the sequence of OvHV-2 DNA isolated from a lymphoblastoid cell line derived from a clinically affected cow. The study confirmed the OvHV-2 sequence information determined for the cell line-isolated DNA and showed no apparently significant changes in the OvHV-2 genome during passage through a clinically susceptible species with subsequent maintenance in vitro. Amino acid identity between the predicted open reading frames (ORFs) of the two genomes was 94-100 %, except for ORF73, which had an identity of 83 %. Polymorphism in ORF73 was due primarily to variability in the G/E-rich repetitive central region of the ORF.
ISSN:0022-1317
1465-2099
DOI:10.1099/vir.0.82285-0