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Impact of Antibiotics on Expression of Virulence-Associated Exotoxin Genes in Methicillin-Sensitive and Methicillin-Resistant Staphylococcus aureus
Extracellular protein toxins contribute to the pathogenesis of a wide variety of Staphylococcus aureus infections. The present study investigated the effects that cell-wall active antibiotics and protein-synthesis inhibitors have on transcription and translation of genes for Panton-Valentine leukoci...
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| Published in: | The Journal of infectious diseases 2007-01, Vol.195 (2), p.202-211 |
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| container_end_page | 211 |
| container_issue | 2 |
| container_start_page | 202 |
| container_title | The Journal of infectious diseases |
| container_volume | 195 |
| creator | Stevens, Dennis L. Ma, Yongsheng Salmi, Daniel B. McIndoo, Eric Wallace, Randi J. Bryant, Amy E. |
| description | Extracellular protein toxins contribute to the pathogenesis of a wide variety of Staphylococcus aureus infections. The present study investigated the effects that cell-wall active antibiotics and protein-synthesis inhibitors have on transcription and translation of genes for Panton-Valentine leukocidin, alpha-hemolysin, and toxic-shock syndrome toxin 1, in both methicillin-sensitive and methicillin-resistant S. aureus Subinhibitory concentrations of nafcillin induced and prolonged mRNA for Panton-Valentine leukocidin, alpha-toxin, and toxic-shock syndrome toxin 1 and increased toxin production. In contrast, clindamycin and linezolid markedly suppressed translation, but not transcription, of toxin genes. These results suggest (1) that protein-synthesis inhibition is an important consideration in the selection of antimicrobial agents to treat serious infections caused by toxin-producing gram-positive pathogens and (2) that, by inducing and enhancing toxin production, inadvertent use of beta-lactam antibiotics to treat methicillin-resistant S. aureus infections may contribute to worse outcomes |
| doi_str_mv | 10.1086/510396 |
| format | article |
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The present study investigated the effects that cell-wall active antibiotics and protein-synthesis inhibitors have on transcription and translation of genes for Panton-Valentine leukocidin, alpha-hemolysin, and toxic-shock syndrome toxin 1, in both methicillin-sensitive and methicillin-resistant S. aureus Subinhibitory concentrations of nafcillin induced and prolonged mRNA for Panton-Valentine leukocidin, alpha-toxin, and toxic-shock syndrome toxin 1 and increased toxin production. In contrast, clindamycin and linezolid markedly suppressed translation, but not transcription, of toxin genes. These results suggest (1) that protein-synthesis inhibition is an important consideration in the selection of antimicrobial agents to treat serious infections caused by toxin-producing gram-positive pathogens and (2) that, by inducing and enhancing toxin production, inadvertent use of beta-lactam antibiotics to treat methicillin-resistant S. aureus infections may contribute to worse outcomes</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>DOI: 10.1086/510396</identifier><identifier>PMID: 17191165</identifier><identifier>CODEN: JIDIAQ</identifier><language>eng</language><publisher>Chicago, IL: The University of Chicago Press</publisher><subject>Acetamides - pharmacology ; Animals ; Anti-Bacterial Agents - pharmacology ; Antibiotics ; Bacteria ; Bacterial Toxins - biosynthesis ; Bacterial Toxins - genetics ; Bacteriology ; Biological and medical sciences ; Clindamycin - pharmacology ; Enterotoxins - biosynthesis ; Enterotoxins - genetics ; Exotoxins - biosynthesis ; Exotoxins - genetics ; Fundamental and applied biological sciences. 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The present study investigated the effects that cell-wall active antibiotics and protein-synthesis inhibitors have on transcription and translation of genes for Panton-Valentine leukocidin, alpha-hemolysin, and toxic-shock syndrome toxin 1, in both methicillin-sensitive and methicillin-resistant S. aureus Subinhibitory concentrations of nafcillin induced and prolonged mRNA for Panton-Valentine leukocidin, alpha-toxin, and toxic-shock syndrome toxin 1 and increased toxin production. In contrast, clindamycin and linezolid markedly suppressed translation, but not transcription, of toxin genes. These results suggest (1) that protein-synthesis inhibition is an important consideration in the selection of antimicrobial agents to treat serious infections caused by toxin-producing gram-positive pathogens and (2) that, by inducing and enhancing toxin production, inadvertent use of beta-lactam antibiotics to treat methicillin-resistant S. aureus infections may contribute to worse outcomes</description><subject>Acetamides - pharmacology</subject><subject>Animals</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotics</subject><subject>Bacteria</subject><subject>Bacterial Toxins - biosynthesis</subject><subject>Bacterial Toxins - genetics</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Clindamycin - pharmacology</subject><subject>Enterotoxins - biosynthesis</subject><subject>Enterotoxins - genetics</subject><subject>Exotoxins - biosynthesis</subject><subject>Exotoxins - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hemolysin Proteins - biosynthesis</subject><subject>Hemolysin Proteins - drug effects</subject><subject>Hemolysin Proteins - genetics</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>Leukocidins</subject><subject>Leukocidins - biosynthesis</subject><subject>Leukocidins - genetics</subject><subject>Linezolid</subject><subject>Medical sciences</subject><subject>Messenger RNA</subject><subject>Methicillin - pharmacology</subject><subject>Methicillin Resistance</subject><subject>Methicillin resistant staphylococcus aureus</subject><subject>Microbial Sensitivity Tests</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Nafcillin - pharmacology</subject><subject>Oxazolidinones - pharmacology</subject><subject>Protein Synthesis Inhibitors - pharmacology</subject><subject>Rabbits</subject><subject>Staphylococcus</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - drug effects</subject><subject>Staphylococcus aureus - growth & development</subject><subject>Staphylococcus aureus - pathogenicity</subject><subject>Superantigens - biosynthesis</subject><subject>Superantigens - drug effects</subject><subject>Superantigens - genetics</subject><subject>Toxicity</subject><subject>Toxins</subject><subject>Vancomycin - pharmacology</subject><subject>Virulence</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNp10M1u1DAUBWALgehQ4A1AYQG7gH9ix1lOq9IpKgK1gKpuLMe5UV0ydvB10PQ5eGFSzagjFqx8pfPpWDqEvGT0PaNafZCMikY9IgsmRV0qxcRjsqCU85LppjkgzxBvKaWVUPVTcsBq1jCm5IL8OVuP1uUi9sUyZN_6mL3DIobiZDMmQPTzOYc_fJoGCA7KJWJ03mboZhJz3PhQnEIALObjM-Qb7_ww-FBeQkCf_W8obOj-SS4APWYbcnGZ7XhzN0QXnZuwsFOCCZ-TJ70dEF7s3kPy_ePJt-NVef7l9Ox4eV66ile51FRJzSnUTPa2tl3T0qq3oobaAWuF0lXTcVnRVjLeS6dbqNqGUy5Au051ThySd9veMcVfE2A2a48OhsEGiBMa1mgpK6730KWImKA3Y_Jrm-4Mo-Z-frOdf4avd41Tu4Zuz3Z7z-DtDlh0duiTDc7j3ulKNFyI2b3ZujiN___s1dbcYo7pQQk6G6rvO8ptPm8Nm4fcpp9G1aKWZnV1ba6-cn5xtLo2n8RfpDGx_w</recordid><startdate>20070115</startdate><enddate>20070115</enddate><creator>Stevens, Dennis L.</creator><creator>Ma, Yongsheng</creator><creator>Salmi, Daniel B.</creator><creator>McIndoo, Eric</creator><creator>Wallace, Randi J.</creator><creator>Bryant, Amy E.</creator><general>The University of Chicago Press</general><general>University of Chicago Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20070115</creationdate><title>Impact of Antibiotics on Expression of Virulence-Associated Exotoxin Genes in Methicillin-Sensitive and Methicillin-Resistant Staphylococcus aureus</title><author>Stevens, Dennis L. ; Ma, Yongsheng ; Salmi, Daniel B. ; McIndoo, Eric ; Wallace, Randi J. ; Bryant, Amy E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c424t-8065820e715fa7ad9b04fa37e7ce1b36849d2540b512f5c8be4b92023e8cd6dc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Acetamides - pharmacology</topic><topic>Animals</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibiotics</topic><topic>Bacteria</topic><topic>Bacterial Toxins - biosynthesis</topic><topic>Bacterial Toxins - genetics</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Clindamycin - pharmacology</topic><topic>Enterotoxins - biosynthesis</topic><topic>Enterotoxins - genetics</topic><topic>Exotoxins - biosynthesis</topic><topic>Exotoxins - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hemolysin Proteins - biosynthesis</topic><topic>Hemolysin Proteins - drug effects</topic><topic>Hemolysin Proteins - genetics</topic><topic>Infections</topic><topic>Infectious diseases</topic><topic>Leukocidins</topic><topic>Leukocidins - biosynthesis</topic><topic>Leukocidins - genetics</topic><topic>Linezolid</topic><topic>Medical sciences</topic><topic>Messenger RNA</topic><topic>Methicillin - pharmacology</topic><topic>Methicillin Resistance</topic><topic>Methicillin resistant staphylococcus aureus</topic><topic>Microbial Sensitivity Tests</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Nafcillin - pharmacology</topic><topic>Oxazolidinones - pharmacology</topic><topic>Protein Synthesis Inhibitors - pharmacology</topic><topic>Rabbits</topic><topic>Staphylococcus</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus aureus - drug effects</topic><topic>Staphylococcus aureus - growth & development</topic><topic>Staphylococcus aureus - pathogenicity</topic><topic>Superantigens - biosynthesis</topic><topic>Superantigens - drug effects</topic><topic>Superantigens - genetics</topic><topic>Toxicity</topic><topic>Toxins</topic><topic>Vancomycin - pharmacology</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stevens, Dennis L.</creatorcontrib><creatorcontrib>Ma, Yongsheng</creatorcontrib><creatorcontrib>Salmi, Daniel B.</creatorcontrib><creatorcontrib>McIndoo, Eric</creatorcontrib><creatorcontrib>Wallace, Randi J.</creatorcontrib><creatorcontrib>Bryant, Amy E.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>The Journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stevens, Dennis L.</au><au>Ma, Yongsheng</au><au>Salmi, Daniel B.</au><au>McIndoo, Eric</au><au>Wallace, Randi J.</au><au>Bryant, Amy E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Impact of Antibiotics on Expression of Virulence-Associated Exotoxin Genes in Methicillin-Sensitive and Methicillin-Resistant Staphylococcus aureus</atitle><jtitle>The Journal of infectious diseases</jtitle><stitle>The Journal of Infectious Diseases</stitle><addtitle>The Journal of Infectious Diseases</addtitle><date>2007-01-15</date><risdate>2007</risdate><volume>195</volume><issue>2</issue><spage>202</spage><epage>211</epage><pages>202-211</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><coden>JIDIAQ</coden><abstract>Extracellular protein toxins contribute to the pathogenesis of a wide variety of Staphylococcus aureus infections. The present study investigated the effects that cell-wall active antibiotics and protein-synthesis inhibitors have on transcription and translation of genes for Panton-Valentine leukocidin, alpha-hemolysin, and toxic-shock syndrome toxin 1, in both methicillin-sensitive and methicillin-resistant S. aureus Subinhibitory concentrations of nafcillin induced and prolonged mRNA for Panton-Valentine leukocidin, alpha-toxin, and toxic-shock syndrome toxin 1 and increased toxin production. In contrast, clindamycin and linezolid markedly suppressed translation, but not transcription, of toxin genes. These results suggest (1) that protein-synthesis inhibition is an important consideration in the selection of antimicrobial agents to treat serious infections caused by toxin-producing gram-positive pathogens and (2) that, by inducing and enhancing toxin production, inadvertent use of beta-lactam antibiotics to treat methicillin-resistant S. aureus infections may contribute to worse outcomes</abstract><cop>Chicago, IL</cop><pub>The University of Chicago Press</pub><pmid>17191165</pmid><doi>10.1086/510396</doi><tpages>10</tpages></addata></record> |
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| ispartof | The Journal of infectious diseases, 2007-01, Vol.195 (2), p.202-211 |
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| subjects | Acetamides - pharmacology Animals Anti-Bacterial Agents - pharmacology Antibiotics Bacteria Bacterial Toxins - biosynthesis Bacterial Toxins - genetics Bacteriology Biological and medical sciences Clindamycin - pharmacology Enterotoxins - biosynthesis Enterotoxins - genetics Exotoxins - biosynthesis Exotoxins - genetics Fundamental and applied biological sciences. Psychology Hemolysin Proteins - biosynthesis Hemolysin Proteins - drug effects Hemolysin Proteins - genetics Infections Infectious diseases Leukocidins Leukocidins - biosynthesis Leukocidins - genetics Linezolid Medical sciences Messenger RNA Methicillin - pharmacology Methicillin Resistance Methicillin resistant staphylococcus aureus Microbial Sensitivity Tests Microbiology Miscellaneous Nafcillin - pharmacology Oxazolidinones - pharmacology Protein Synthesis Inhibitors - pharmacology Rabbits Staphylococcus Staphylococcus aureus Staphylococcus aureus - drug effects Staphylococcus aureus - growth & development Staphylococcus aureus - pathogenicity Superantigens - biosynthesis Superantigens - drug effects Superantigens - genetics Toxicity Toxins Vancomycin - pharmacology Virulence |
| title | Impact of Antibiotics on Expression of Virulence-Associated Exotoxin Genes in Methicillin-Sensitive and Methicillin-Resistant Staphylococcus aureus |
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