Analyses of Promoter Hypermethylation for RUNX3 and other Tumor Suppressor Genes in Nasopharyngeal Carcinoma

Background: Aberrant methylation of cytosine in promoter CpG islands is a recognized contributory process to carcinogenesis. This study explores the methylation profile of RUNX3 in combination with p16, RASSF1A, CDH1 and hMLH1 in nasopharyngeal carcinoma (NPC) patients. Materials and Methods: Genomi...

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Bibliographic Details
Published in:Anticancer research 2006-11, Vol.26 (6B), p.4287-4292
Main Authors: TAN, Sing-Huang, IDA, Hiroshi, GOH, Boon-Cher, HSIEH, Wenson, LOH, Marie, ITO, Yoshiaki
Format: Article
Language:English
Subjects:
Base Sequence
Biological and medical sciences
Core Binding Factor Alpha 3 Subunit - genetics
DNA Primers
Genes, Tumor Suppressor
Humans
Male
Medical sciences
Nasopharyngeal Neoplasms - genetics
Otorhinolaryngology. Stomatology
Polymerase Chain Reaction
Promoter Regions, Genetic
Tumors
Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology
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Summary:Background: Aberrant methylation of cytosine in promoter CpG islands is a recognized contributory process to carcinogenesis. This study explores the methylation profile of RUNX3 in combination with p16, RASSF1A, CDH1 and hMLH1 in nasopharyngeal carcinoma (NPC) patients. Materials and Methods: Genomic DNA was extracted from 19 fresh frozen NPC biopsies, which were then subjected to bisulfite conversion and methylation-specific PCR for analysis of promoter hypermethylation for the five respective genes. Three cell lines, SNU1, RKO and LS174T, were used as controls. Results: The incidences of promoter methylation were as follows: RUNX3 0/19 (0%), p16 6/19 (32%), RASSF1A 13/19 (68%), CDH1 9/19 (47%) and hMLH1 4/19 (21%). Ninety-five percent of the tumor specimens displayed aberrant methylation in at least one of these genes. No significant correlation between methylation status of these genes and clinical parameters was found. Conclusion: Methylation of multiple genes is involved in critical pathways for cancer development in NPC. Promoter hypermethylation for RUNX3 was, however, not present.
ISSN:0250-7005
1791-7530