Recombinant Truncated Cry1Ca Protein Is Toxic to Lepidopteran Insects and Forms Large Cuboidal Crystals in Insect Cells

A truncated version of the cry1Ca gene from Bacillus thuringiensis was introduced into the genome of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) under the control of two promoters. A recombinant virus (vSyncry1c) was isolated and used to infect insect cells in culture and insect la...

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Bibliographic Details
Published in:Current microbiology 2006-10, Vol.53 (4), p.287-292
Main Authors: Aguiar, Raimundo W. S, Martins, Érica S, Valicente, Fernando H, Carneiro, Newton P, Batista, Andréia C, Melatti, Viviane M, Monnerat, Rose G, Ribeiro, Bergmann M
Format: Article
Language:English
Subjects:
Animals
Autographa californica
Bacillus thuringiensis
Bacterial Proteins - genetics
Bacterial Proteins - toxicity
Bacterial Toxins - genetics
Bacterial Toxins - toxicity
Bioassays
Crystals
Endotoxins - genetics
Endotoxins - toxicity
Genes
Hemolysin Proteins
Insects
Larva - virology
Larvae
Lepidoptera
Lepidoptera - ultrastructure
Lepidoptera - virology
Nuclear polyhedrosis virus
Nucleopolyhedrovirus - genetics
Pest Control, Biological
Proteins
Recombinant Proteins - toxicity
Spodoptera - ultrastructure
Spodoptera - virology
Toxins
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Summary:A truncated version of the cry1Ca gene from Bacillus thuringiensis was introduced into the genome of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) under the control of two promoters. A recombinant virus (vSyncry1c) was isolated and used to infect insect cells in culture and insect larvae. Structural and ultrastructural analysis of insects infected with vSyncry1C showed the formation of large cuboidal crystals inside the cytoplasm of insect cells in culture and in insect cadavers late in infection. Infected insect cell extracts were analyzed by SDS-PAGE and Western blot and showed the presence of a 65-kDa polypeptide probably corresponding to the protease processed form of the toxin. Bioassays using purified recombinant toxin crystals showed a CL₅₀ of 19.49 ng/ml for 2nd instar A. gemmatalis larvae and 114.1 ng/ml for S. frugiperda.
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-005-0502-3