Reusing ethyl acetate and aqueous exhausted fractions of dry olive mill residue by saprobe fungi

• Published in: Chemosphere (Oxford) 2007, Vol.66 (1), p.67-74
• Main Authors: Aranda, E., García-Romera, I., Ocampo, J.A., Carbone, V., Malorni, A., Sannino, F., De Martino, A., Capasso, R.
• Format: Article
• Language: English
• Subjects: acetates; Acetates - chemistry; Acetates - metabolism; Agriculture, rearing and food industries wastes; Agronomy. Soil science and plant productions; antioxidants; Applied sciences; aqueous exhausted fraction; Basidiomycota; Basidiomycota - growth & development; Basidiomycota - metabolism; bioassays; Biodegradation, Environmental; Biofertilizer; biofertilizers; Biological and medical sciences; biological treatment; Biological treatment of sewage sludges and wastes; Biotechnology; biotransformation; Chromatography, High Pressure Liquid; decay fungi; Dry olive mill residue; dry olive mill residues; Environment and pollution; ethyl acetate fraction; Exact sciences and technology; extraction; extracts; Food industries; food processing wastes; Fundamental and applied biological sciences. Psychology; General agronomy. Plant production; Germination - drug effects; hydroxytyrosol; Industrial applications and implications. Economical aspects; Industrial Waste - analysis; Industrial Waste - prevention & control; Laccase - metabolism; Lepidium sativum; Lepidium sativum - drug effects; Mass spectrometry; metabolic detoxification; Molecular Structure; Olea; Olive Oil; olives; Other nutrients. Amendments. Solid and liquid wastes. Sludges and slurries; Phenols; Phenols - metabolism; Phlebia radiata; Phytotoxicity; Plant Oils - chemistry; Plant Oils - metabolism; Pleurotus pulmonarius; Pollution; Pycnoporus cinnabarinus; Saprobe fungi; seed germination; Seeds - drug effects; Soil-plant relationships. Soil fertility. Fertilization. Amendments; Spectrometry, Mass, Electrospray Ionization; Trametes versicolor; Use and upgrading of agricultural and food by-products. Biotechnology; waste utilization; Wastes; Water Pollutants, Chemical - metabolism; Water Pollutants, Chemical - toxicity
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2006.05.021

Some saprobe fungi ( Phlebia radiata, Trametes versicolor, Coriolopsis rigida, Pycnoporus cinnabarinus, Fomes sclerodermus or Pleurotus pulmonarius) were able to bioconvert the ethyl acetate fraction (DEAF) and the corresponding aqueous exhausted fraction (EAF) of dry olive mill residue (DOR), reducing their phytotoxicity on Lepidium sativum seeds. Large amount of hydroxytyrosol together with other eight monomeric phenols were found in the native DEAF fraction, which represents a good source of antioxidants. P. radiata, T. versicolor and F. sclerodermus caused an effective phytotoxicity reduction of EAF in the concentration range of 25–3 g l −1. In particular, in the range between 12.5 and 3 g l −1, the EAF samples inoculated with P. radiata and F. sclerodermus surprisingly stimulated the germinability of L. sativum, suggesting their use as a potential biofertilizer. This is the first report which showed the bioconversion of the above fractions in shorter time with respect to the previous findings concerning DOR. The possible implications of laccase in the decrease of DEAF and EAF phytotoxicity was also discussed.