Cloning and Characterization of a 4-Hydroxyphenylacetate 3-Hydroxylase From the Thermophile Geobacillus sp. PA-9

A 4-hydroxyphenylacetic acid (4-HPA) hydroxylase-encoding gene, on a 2.7-kb genomic DNA fragment, was cloned from the thermophile Geobacillus sp. PA-9. The Geobacillus sp. PA-9 4-HPA hydroxylase gene, designated hpaH, encodes a protein of 494 amino acids with a predicted molecular mass of 56.269 Da....

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Bibliographic Details
Published in:Current microbiology 2007-12, Vol.55 (6), p.480-484
Main Authors: Hawumba, J. F, Brözel, V. S, Theron, J
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
Arthrobacter globiformis
Bacillaceae - enzymology
Bacillaceae - genetics
Bacillaceae - growth & development
Bacteria
Cloning
Cloning, Molecular
Deoxyribonucleic acid
DNA
DNA, Bacterial - analysis
DNA, Bacterial - isolation & purification
E coli
Enzymes
Escherichia coli
Geobacillus
Klebsiella pneumoniae
Microbiology
Mixed Function Oxygenases - chemistry
Mixed Function Oxygenases - genetics
Mixed Function Oxygenases - metabolism
Molecular Sequence Data
Phenylacetates - metabolism
Sequence Alignment
Sequence Analysis, DNA
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Summary:A 4-hydroxyphenylacetic acid (4-HPA) hydroxylase-encoding gene, on a 2.7-kb genomic DNA fragment, was cloned from the thermophile Geobacillus sp. PA-9. The Geobacillus sp. PA-9 4-HPA hydroxylase gene, designated hpaH, encodes a protein of 494 amino acids with a predicted molecular mass of 56.269 Da. The deduced amino-acid sequence of the hpaH gene product displayed
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-007-9016-5