Production, purification and partial characterization of lipase from Trichoderma Viride

A new strain of lipolytic Trichoderma viride was isolated from the soil on a selective medium that contained olive oil as the only source of carbon and energy. The isolated strain was cultivated for lipase production in shake flasks at 30 plus or minus 1 degree C and the fermentation pattern was stu...

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Bibliographic Details
Published in:African journal of biotechnology 2006-05, Vol.5 (10), p.878-882
Main Authors: Kashmiri, MA, Adnan, A, Butt, B W
Format: Article
Language:English
Subjects:
Olea
Trichoderma viride
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Summary:A new strain of lipolytic Trichoderma viride was isolated from the soil on a selective medium that contained olive oil as the only source of carbon and energy. The isolated strain was cultivated for lipase production in shake flasks at 30 plus or minus 1 degree C and the fermentation pattern was studied. The maximum extracellular lipase activity of 7.3 U/mL and the maximum intracellular activity of 320 U/g mycelium were noted after 48 h. Although maximum fungal biomass was present at 13.6 g/L at 60 h but highest specific growth rate was observed between 6 and 18 h. The extracellular lipase present in the broth was, purified 134-folds with an overall yield of 46% through purification procedure of ammonium sulphate precipitation, ion exchange and gel permeation chromatography. The K sub(m) value of the purified enzyme for triolein hydrolysis was found to be 1.229 m.mole/L.
ISSN:1684-5315
1684-5315