Fast real-time polymerase chain reaction for quantitative detection of Lactobacillus delbrueckii bacteriophages in milk

One of the main microbiological problems of the dairy industry is the susceptibility of starter bacteria to virus infections. Lactobacillus delbrueckii, a component of thermophilic starter cultures used in the manufacture of several fermented dairy products, including yogurt, is also sensitive to ba...

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Bibliographic Details
Published in:Food microbiology 2008-12, Vol.25 (8), p.978-982
Main Authors: Martín, Maria Cruz, del Rio, Beatriz, Martínez, Noelia, Magadán, Alfonso H., Alvarez, Miguel A.
Format: Article
Language:English
Subjects:
accuracy
Animals
Bacteria
Bacteriophage detection
bacteriophages
Bacteriophages - isolation & purification
Biological and medical sciences
Cattle
Consumer Product Safety
detection
Fermentation
food contamination
Food Contamination - analysis
Food industries
Food microbiology
Fundamental and applied biological sciences. Psychology
General aspects
Humans
Lactobacillus delbrueckii
Lactobacillus delbrueckii - virology
Methods of analysis, processing and quality control, regulation, standards
microbial contamination
microbial genetics
milk
Milk - virology
molecular sequence data
nucleotide sequences
polymerase chain reaction
Polymerase Chain Reaction - methods
precision
quantitative analysis
Real-time quantitative PCR
Sensitivity and Specificity
starter cultures
test sensitivity
test specificity
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Summary:One of the main microbiological problems of the dairy industry is the susceptibility of starter bacteria to virus infections. Lactobacillus delbrueckii, a component of thermophilic starter cultures used in the manufacture of several fermented dairy products, including yogurt, is also sensitive to bacteriophage attacks. To avoid the problems associated with these viruses, quick and sensitive detection methods are necessary. In the present study, a fast real-time quantitative polymerase chain reaction assay for the direct detection and quantification of L. delbrueckii phages in milk was developed. A set of primers and a TaqMan MGB probe was designed, based on the lysin gene sequence of different L. delbrueckii phages. The results show the proposed method to be a rapid (total processing time 30 min), specific and highly sensitive technique for detecting L. delbrueckii phages in milk.
ISSN:0740-0020
1095-9998
DOI:10.1016/j.fm.2008.07.004