The ellagitannin HeT induces electrolyte leakage, calcium influx and the accumulation of nitric oxide and hydrogen peroxide in strawberry

HeT (1-0-galloyl-2,3; 4,6-bis-hexahydroxydiphenoyl-β-D-glucopyranose) is a penta-esterified ellagitannin obtained from strawberry leaves. Previous studies have shown that foliar application of HeT prior to inoculation with a virulent pathogen increases the resistance toward Colletotrichum acutatum i...

Full description

Saved in:
Bibliographic Details
Published in:Plant physiology and biochemistry 2018-02, Vol.123, p.400-405
Main Authors: Martos, Gustavo Gabriel, Mamaní, Alicia, Filippone, María Paula, Castagnaro, Atilio Pedro, Díaz Ricci, Juan Carlos
Format: Article
Language:English
Subjects:
Calcium - metabolism
Calcium Signaling
Defense
Electrolytes - metabolism
Elicitor
Ellagitannin
Fragaria - metabolism
Fragaria - microbiology
Hydrogen Peroxide - metabolism
Hydrolyzable Tannins - pharmacology
Nitric Oxide - metabolism
Plant Diseases - microbiology
Signaling
Strawberry
Xanthomonas - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:HeT (1-0-galloyl-2,3; 4,6-bis-hexahydroxydiphenoyl-β-D-glucopyranose) is a penta-esterified ellagitannin obtained from strawberry leaves. Previous studies have shown that foliar application of HeT prior to inoculation with a virulent pathogen increases the resistance toward Colletotrichum acutatum in strawberry plants and to Xanthomonas citri subsp. citri in lemon plants. In this work we report that HeT induces an immediate leak of electrolytes, the hyperpolarization of the cellular membrane, a rapid Ca2+ influx to the cytoplasm during the first few seconds, which in turn modulates the accumulation of nitric oxide 5 min after treatment. At longer times, a biphasic accumulation of H2O2 with peaks at 2 and 5 h post treatment could be observed. In addition, HeT elicited the increase of alternative oxidase capacity during the first 12 h post treatment. •HeT induces a rapid electrolyte leakage and hyperpolarization on strawberry cells.•HeT produces the Ca2+ influx which inhibits the nitric oxide accumulation.•HeT causes a biphasic accumulation of H2O2 and the AOX activation.
ISSN:0981-9428
1873-2690
DOI:10.1016/j.plaphy.2017.12.035