miR-137 inhibits melanoma cell proliferation through downregulation of GLO1

Late-stage melanoma is refractory to current therapies. MicroRNAs (miRNAs) can modulate many physiological and pathological processes of melanoma. Studies have demonstrated that miR-137 acts as a tumor suppressor by inhibiting the proliferation of melanoma cells through targeting multiple mRNAs. The...

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Published in:Science China. Life sciences 2018-05, Vol.61 (5), p.541-549
Main Authors: Lv, Na, Hao, Shuai, Luo, Chonglin, Abukiwan, Alia, Hao, Ying, Gai, Fei, Huang, Weiwei, Huang, Lingyun, Xiao, Xueyuan, Eichmüller, Stefan B., He, Dacheng
Format: Article
Language:English
Subjects:
3' Untranslated Regions
Bioinformatics
Biomedical and Life Sciences
Cell growth
Cell Line, Tumor
Cell proliferation
Cell Proliferation - physiology
Gene Expression Regulation, Neoplastic - genetics
Gene Knockdown Techniques
Glycolysis
Humans
Kinases
Labeling
Lactoylglutathione Lyase - genetics
Life Sciences
Melanoma
Melanoma - metabolism
Melanoma - pathology
MicroRNAs - metabolism
miRNA
Polymerase chain reaction
Proteomics
Pyruvaldehyde
Research Paper
siRNA
Tumor suppressor genes
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Summary:Late-stage melanoma is refractory to current therapies. MicroRNAs (miRNAs) can modulate many physiological and pathological processes of melanoma. Studies have demonstrated that miR-137 acts as a tumor suppressor by inhibiting the proliferation of melanoma cells through targeting multiple mRNAs. The glyoxalase system member glyoxalase 1 (GLO1) is the principal scavenging enzyme of methylglyoxal (MG), a toxic byproduct of glycolysis. Using 35 S in vivo / vitro labelling analysis for dynamic proteomics (SiLAD), we found that miR-137 downregulated the expression of GLO1 in melanoma cells. Bioinformatics analysis predicted that GLO1 is a direct target of miR-137. This was validated by dual luciferase reporter assay. Quantitative RT-PCR (qRT-PCR) and western blot analysis indicated that miR-137 could decrease endogenous GLO1 expression. Furthermore, siRNA targeting of GLO1 mimicked inhibition of melanoma cell proliferation caused by miR-137 overexpression. Re-expression of GLO1 was able to restore miR-137-mediated suppression of melanoma cell proliferation. Therefore, these results suggest that miR-137 inhibits the proliferation of melanoma cells by targeting GLO1.
ISSN:1674-7305
1869-1889
DOI:10.1007/s11427-017-9138-9