Quantification of DNA/SWCNT Solvation Differences by Aqueous Two-Phase Separation

Single-walled carbon nanotubes (SWCNTs) coated with single-stranded DNA can be effectively separated into various chiralities using an aqueous two-phase (ATP) system. Partitioning is driven by small differences in the dissolution characteristics of the hybrid between the two phases. Thus, in additio...

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Bibliographic Details
Published in:Langmuir 2018-02, Vol.34 (5), p.1834-1843
Main Authors: Yang, Yoona, Shankar, Akshaya, Aryaksama, Thibault, Zheng, Ming, Jagota, Anand
Format: Article
Language:English
Subjects:
DNA - chemistry
Nanotubes, Carbon - chemistry
Solubility
Solvents - chemistry
Thermodynamics
Water - chemistry
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Summary:Single-walled carbon nanotubes (SWCNTs) coated with single-stranded DNA can be effectively separated into various chiralities using an aqueous two-phase (ATP) system. Partitioning is driven by small differences in the dissolution characteristics of the hybrid between the two phases. Thus, in addition to being a separation technique, the ATP system potentially also offers a way to quantify and rank the dissolution properties of the solute (here the DNA/SWCNT hybrids), such as the solvation free energy and solubility. In this study, we propose two different approaches to quantitatively analyze the ATP partitioning of DNA/SWCNT hybrids. First, we present a model that extracts the relative solvation free energy of various DNA/SWCNT hybrids by using an expansion relative to a standard state. Second, we extract a solubility parameter by analyzing the partitioning of hybrids in the ATP system. The two approaches are found to be consistent, providing some confidence in each as a method of quantifying differences in the solubility of various DNA/SWCNT hybrids.
ISSN:0743-7463
1520-5827
DOI:10.1021/acs.langmuir.7b03186