Protective efficacy of a DNA vaccine encoding capsid protein of porcine circovirus‐like virus P1 against porcine circovirus 2 in mice

ABSTRACT The capsid protein is the major immunogenic protein of porcine circovirus 2 (PCV2). The nucleotide sequence of porcine circovirus‐like virus P1 shares high homology with open reading frame (ORF) 2 of PCV2, and ORF1 of P1 encodes its structural protein. Mice were vaccinated twice intramuscul...

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Bibliographic Details
Published in:Microbiology and immunology 2018-03, Vol.62 (3), p.195-199
Main Authors: Zhang, Dan, He, Kongwang, Wen, Libin, Fan, Hongjie
Format: Article
Language:English
Subjects:
Capsid protein
DNA vaccine
DNA vaccines
Homology
Immune response
Immunogenicity
Mice
Nucleotide sequence
porcine circovirus 2
porcine circovirus‐like virus P1
Proteins
Vaccines
Viremia
Viruses
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Summary:ABSTRACT The capsid protein is the major immunogenic protein of porcine circovirus 2 (PCV2). The nucleotide sequence of porcine circovirus‐like virus P1 shares high homology with open reading frame (ORF) 2 of PCV2, and ORF1 of P1 encodes its structural protein. Mice were vaccinated twice intramuscularly with a plasmid expressing the P1 ORF1 protein (pcDNA3.1(+)‐ORF1) at 2‐week intervals. All animals vaccinated with pcDNA3.1(+)‐ORF1 developed higher specific anti‐P1 antibody levels, and had less PCV2 viremia and milder histopathological changes than PCV2‐challenged mice in the control group. Our results show that the P1 DNA vaccine elicited immune responses against PCV2 infection in a mouse model.
ISSN:0385-5600
1348-0421
DOI:10.1111/1348-0421.12571