An improved design to capture magnetic microparticles for capillary electrophoresis based immobilized microenzyme reactors

In this paper, we demonstrate the effectiveness of a new 3D printed magnet holder that enables capture of magnetic microparticles in commercially available capillary electrophoresis equipment with a liquid or air based coolant system. The design as well as the method to capture magnetic microparticl...

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Bibliographic Details
Published in:Electrophoresis 2018-04, Vol.39 (7), p.981-988
Main Authors: Ramana, Pranov, Schejbal, Jan, Houthoofd, Kristof, Martens, Johan, Adams, Erwin, Augustijns, Patrick, Glatz, Zdenĕk, Schepdael, Ann
Format: Article
Language:English
Subjects:
Capillary electrophoresis
Electrophoresis
Enzyme activity
Glutaraldehyde
IMER
Immobilization
In‐line capillary electrophoresis
Magnetic microparticles
Magnetism
Microparticles
Reactors
Serum albumin
Substrates
Three dimensional printing
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Summary:In this paper, we demonstrate the effectiveness of a new 3D printed magnet holder that enables capture of magnetic microparticles in commercially available capillary electrophoresis equipment with a liquid or air based coolant system. The design as well as the method to capture magnetic microparticles inside the capillary are discussed. This setup was tested at temperature and pH values suitable for performing enzymatic reactions. To demonstrate its applicability in CE‐ immobilized microenzyme reactors (IMER) development, human flavin‐containing monooxygenase 3 and bovine serum albumin were immobilized on amino functionalized magnetic microparticles using glutaraldehyde. These microparticles were subsequently used to perform in‐line capillary electrophoresis with clozapine as a model substrate. This setup could be used further to establish CE‐IMERs of other drug metabolic enzymes in a commercially available liquid based capillary coolant system. The CE‐IMER setup was successful, although a subsequent decrease in enzyme activity was observed on repeated runs.
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.201700434