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EGF is highly expressed in hepatocellular carcinoma (HCC) and promotes motility of HCC cells via fibronectin
Better understanding of metastasis process would allow for the development of effective approaches to treat hepatocellular carcinoma (HCC). Recent literature has highlighted the fundamental role of interaction between tumor cells and their microenvironment components in tumor metastasis. Aberrant ex...
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Published in: | Journal of cellular biochemistry 2018-05, Vol.119 (5), p.4170-4183 |
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description | Better understanding of metastasis process would allow for the development of effective approaches to treat hepatocellular carcinoma (HCC). Recent literature has highlighted the fundamental role of interaction between tumor cells and their microenvironment components in tumor metastasis. Aberrant expression of epidermal growth factor (EGF) induces highly malignant HCC, and activated EGF/EGFR signaling is correlated with an aggressive phenotype and intrahepatic metastasis. Thus, EGF in the tumor microenvironment may influence the behavior of HCC cells. In this study, for the first time, we studied the expression of EGF in HCCs, and the potential role of EGF in the motility of HCC cells and the underlying mechanisms. It was demonstrated that EGF was highly expressed in HCCs and positively associated with higher tumor grade. In addition, EGF promoted the migration and invasion of HCC cells mainly via induction of fibronectin (FN) in vitro. Mechanistically, EGF simultaneously increased the nuclear translocation and PKC mediated phosphorylation of p65 which could bind to the −356 bp to −259 bp fragment of FN promoter, leading to a markedly increased activity of FN promoter in HCC cells. These results highlight the potential role of EGF in promoting HCC metastasis, demonstrate a novel pathway for regulation of FN expression and provide potential targets for HCC prevention and treatment.
Epidermal growth factor (EGF) was highly expressed in hepatocellular carcinoma (HCCs), and positively associated with higher tumor grade. In vitro, EGF promoted the migration and invasion of HCC cells mainly via induction of fibronectin (FN). |
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Epidermal growth factor (EGF) was highly expressed in hepatocellular carcinoma (HCCs), and positively associated with higher tumor grade. In vitro, EGF promoted the migration and invasion of HCC cells mainly via induction of fibronectin (FN).</description><identifier>ISSN: 0730-2312</identifier><identifier>EISSN: 1097-4644</identifier><identifier>DOI: 10.1002/jcb.26625</identifier><identifier>PMID: 29315755</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Carcinoma, Hepatocellular - genetics ; Carcinoma, Hepatocellular - metabolism ; Carcinoma, Hepatocellular - pathology ; cell motility ; Cell Movement ; Epidermal growth factor ; epidermal growth factor (EGF) ; Epidermal Growth Factor - biosynthesis ; Epidermal Growth Factor - genetics ; Epidermal growth factor receptors ; Fibronectin ; Fibronectins - genetics ; Fibronectins - metabolism ; Gene Expression Regulation, Neoplastic ; Hep G2 Cells ; Hepatocellular carcinoma ; hepatocellular carcinoma (HCC) ; Humans ; Liver cancer ; Liver Neoplasms - genetics ; Liver Neoplasms - metabolism ; Liver Neoplasms - pathology ; Metastases ; Metastasis ; Motility ; Neoplasm Proteins - genetics ; Neoplasm Proteins - metabolism ; Nuclear transport ; Phenotypes ; Phosphorylation ; Protein kinase C ; Signaling ; Translocation ; Tumor cells ; tumor microenvironment</subject><ispartof>Journal of cellular biochemistry, 2018-05, Vol.119 (5), p.4170-4183</ispartof><rights>2018 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3535-b0c0f2f270763cd47df1d3ab8b1f1981d9a8bc07032ed7b8f0220ade1e4558c53</citedby><cites>FETCH-LOGICAL-c3535-b0c0f2f270763cd47df1d3ab8b1f1981d9a8bc07032ed7b8f0220ade1e4558c53</cites><orcidid>0000-0003-4872-0843</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29315755$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Zongcai</creatorcontrib><creatorcontrib>Chen, Danyang</creatorcontrib><creatorcontrib>Ning, Fen</creatorcontrib><creatorcontrib>Du, Jun</creatorcontrib><creatorcontrib>Wang, Haifang</creatorcontrib><title>EGF is highly expressed in hepatocellular carcinoma (HCC) and promotes motility of HCC cells via fibronectin</title><title>Journal of cellular biochemistry</title><addtitle>J Cell Biochem</addtitle><description>Better understanding of metastasis process would allow for the development of effective approaches to treat hepatocellular carcinoma (HCC). Recent literature has highlighted the fundamental role of interaction between tumor cells and their microenvironment components in tumor metastasis. Aberrant expression of epidermal growth factor (EGF) induces highly malignant HCC, and activated EGF/EGFR signaling is correlated with an aggressive phenotype and intrahepatic metastasis. Thus, EGF in the tumor microenvironment may influence the behavior of HCC cells. In this study, for the first time, we studied the expression of EGF in HCCs, and the potential role of EGF in the motility of HCC cells and the underlying mechanisms. It was demonstrated that EGF was highly expressed in HCCs and positively associated with higher tumor grade. In addition, EGF promoted the migration and invasion of HCC cells mainly via induction of fibronectin (FN) in vitro. Mechanistically, EGF simultaneously increased the nuclear translocation and PKC mediated phosphorylation of p65 which could bind to the −356 bp to −259 bp fragment of FN promoter, leading to a markedly increased activity of FN promoter in HCC cells. These results highlight the potential role of EGF in promoting HCC metastasis, demonstrate a novel pathway for regulation of FN expression and provide potential targets for HCC prevention and treatment.
Epidermal growth factor (EGF) was highly expressed in hepatocellular carcinoma (HCCs), and positively associated with higher tumor grade. In vitro, EGF promoted the migration and invasion of HCC cells mainly via induction of fibronectin (FN).</description><subject>Carcinoma, Hepatocellular - genetics</subject><subject>Carcinoma, Hepatocellular - metabolism</subject><subject>Carcinoma, Hepatocellular - pathology</subject><subject>cell motility</subject><subject>Cell Movement</subject><subject>Epidermal growth factor</subject><subject>epidermal growth factor (EGF)</subject><subject>Epidermal Growth Factor - biosynthesis</subject><subject>Epidermal Growth Factor - genetics</subject><subject>Epidermal growth factor receptors</subject><subject>Fibronectin</subject><subject>Fibronectins - genetics</subject><subject>Fibronectins - metabolism</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Hep G2 Cells</subject><subject>Hepatocellular carcinoma</subject><subject>hepatocellular carcinoma (HCC)</subject><subject>Humans</subject><subject>Liver cancer</subject><subject>Liver Neoplasms - genetics</subject><subject>Liver Neoplasms - metabolism</subject><subject>Liver Neoplasms - pathology</subject><subject>Metastases</subject><subject>Metastasis</subject><subject>Motility</subject><subject>Neoplasm Proteins - genetics</subject><subject>Neoplasm Proteins - metabolism</subject><subject>Nuclear transport</subject><subject>Phenotypes</subject><subject>Phosphorylation</subject><subject>Protein kinase C</subject><subject>Signaling</subject><subject>Translocation</subject><subject>Tumor cells</subject><subject>tumor microenvironment</subject><issn>0730-2312</issn><issn>1097-4644</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp1kUtP3DAURi1EBcNjwR9AltjAInBtx3GypBGPVkjdlLXl-MF4lMRTO2k7_x4PA10gdXO9uMdHn-6H0BmBawJAb1a6u6ZVRfkeWhBoRFFWZbmPFiAYFJQReoiOUloBQNMweoAOacMIF5wvUH_3cI99wkv_suw32P5dR5uSNdiPeGnXagra9v3cq4i1itqPYVD48rFtr7AaDV7HMITJJpyn7_20wcHhvMXbXwn_9go738UwWj358QR9capP9vT9PUbP93c_28fi6cfDt_b2qdCMM150oMFRRwWIimlTCuOIYaqrO-JIUxPTqLrTIIBRa0RXO6AUlLHElpzXmrNjdLnz5ni_ZpsmOfi0TaRGG-Yks6ThnDcUMnrxCV2FOY45naRAauCkElvh1Y7SMaQUrZPr6AcVN5KA3FYgcwXyrYLMnr8b526w5h_5cfMM3OyAP763m_-b5Pf26075CseCjtM</recordid><startdate>201805</startdate><enddate>201805</enddate><creator>Liu, Zongcai</creator><creator>Chen, Danyang</creator><creator>Ning, Fen</creator><creator>Du, Jun</creator><creator>Wang, Haifang</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-4872-0843</orcidid></search><sort><creationdate>201805</creationdate><title>EGF is highly expressed in hepatocellular carcinoma (HCC) and promotes motility of HCC cells via fibronectin</title><author>Liu, Zongcai ; Chen, Danyang ; Ning, Fen ; Du, Jun ; Wang, Haifang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3535-b0c0f2f270763cd47df1d3ab8b1f1981d9a8bc07032ed7b8f0220ade1e4558c53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Carcinoma, Hepatocellular - genetics</topic><topic>Carcinoma, Hepatocellular - metabolism</topic><topic>Carcinoma, Hepatocellular - pathology</topic><topic>cell motility</topic><topic>Cell Movement</topic><topic>Epidermal growth factor</topic><topic>epidermal growth factor (EGF)</topic><topic>Epidermal Growth Factor - biosynthesis</topic><topic>Epidermal Growth Factor - genetics</topic><topic>Epidermal growth factor receptors</topic><topic>Fibronectin</topic><topic>Fibronectins - genetics</topic><topic>Fibronectins - metabolism</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Hep G2 Cells</topic><topic>Hepatocellular carcinoma</topic><topic>hepatocellular carcinoma (HCC)</topic><topic>Humans</topic><topic>Liver cancer</topic><topic>Liver Neoplasms - genetics</topic><topic>Liver Neoplasms - metabolism</topic><topic>Liver Neoplasms - pathology</topic><topic>Metastases</topic><topic>Metastasis</topic><topic>Motility</topic><topic>Neoplasm Proteins - genetics</topic><topic>Neoplasm Proteins - metabolism</topic><topic>Nuclear transport</topic><topic>Phenotypes</topic><topic>Phosphorylation</topic><topic>Protein kinase C</topic><topic>Signaling</topic><topic>Translocation</topic><topic>Tumor cells</topic><topic>tumor microenvironment</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Zongcai</creatorcontrib><creatorcontrib>Chen, Danyang</creatorcontrib><creatorcontrib>Ning, Fen</creatorcontrib><creatorcontrib>Du, Jun</creatorcontrib><creatorcontrib>Wang, Haifang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cellular biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Zongcai</au><au>Chen, Danyang</au><au>Ning, Fen</au><au>Du, Jun</au><au>Wang, Haifang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>EGF is highly expressed in hepatocellular carcinoma (HCC) and promotes motility of HCC cells via fibronectin</atitle><jtitle>Journal of cellular biochemistry</jtitle><addtitle>J Cell Biochem</addtitle><date>2018-05</date><risdate>2018</risdate><volume>119</volume><issue>5</issue><spage>4170</spage><epage>4183</epage><pages>4170-4183</pages><issn>0730-2312</issn><eissn>1097-4644</eissn><abstract>Better understanding of metastasis process would allow for the development of effective approaches to treat hepatocellular carcinoma (HCC). Recent literature has highlighted the fundamental role of interaction between tumor cells and their microenvironment components in tumor metastasis. Aberrant expression of epidermal growth factor (EGF) induces highly malignant HCC, and activated EGF/EGFR signaling is correlated with an aggressive phenotype and intrahepatic metastasis. Thus, EGF in the tumor microenvironment may influence the behavior of HCC cells. In this study, for the first time, we studied the expression of EGF in HCCs, and the potential role of EGF in the motility of HCC cells and the underlying mechanisms. It was demonstrated that EGF was highly expressed in HCCs and positively associated with higher tumor grade. In addition, EGF promoted the migration and invasion of HCC cells mainly via induction of fibronectin (FN) in vitro. Mechanistically, EGF simultaneously increased the nuclear translocation and PKC mediated phosphorylation of p65 which could bind to the −356 bp to −259 bp fragment of FN promoter, leading to a markedly increased activity of FN promoter in HCC cells. These results highlight the potential role of EGF in promoting HCC metastasis, demonstrate a novel pathway for regulation of FN expression and provide potential targets for HCC prevention and treatment.
Epidermal growth factor (EGF) was highly expressed in hepatocellular carcinoma (HCCs), and positively associated with higher tumor grade. In vitro, EGF promoted the migration and invasion of HCC cells mainly via induction of fibronectin (FN).</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>29315755</pmid><doi>10.1002/jcb.26625</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0003-4872-0843</orcidid></addata></record> |
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subjects | Carcinoma, Hepatocellular - genetics Carcinoma, Hepatocellular - metabolism Carcinoma, Hepatocellular - pathology cell motility Cell Movement Epidermal growth factor epidermal growth factor (EGF) Epidermal Growth Factor - biosynthesis Epidermal Growth Factor - genetics Epidermal growth factor receptors Fibronectin Fibronectins - genetics Fibronectins - metabolism Gene Expression Regulation, Neoplastic Hep G2 Cells Hepatocellular carcinoma hepatocellular carcinoma (HCC) Humans Liver cancer Liver Neoplasms - genetics Liver Neoplasms - metabolism Liver Neoplasms - pathology Metastases Metastasis Motility Neoplasm Proteins - genetics Neoplasm Proteins - metabolism Nuclear transport Phenotypes Phosphorylation Protein kinase C Signaling Translocation Tumor cells tumor microenvironment |
title | EGF is highly expressed in hepatocellular carcinoma (HCC) and promotes motility of HCC cells via fibronectin |
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