Levels of oxidative stress biomarkers and bone resorption regulators in apical periodontitis lesions infected by Epstein–Barr virus

• Published in: International endodontic journal 2018-06, Vol.51 (6), p.593-604
• Main Authors: Jakovljevic, A., Andric, M., Nikolic, N., Coric, V., Krezovic, S., Carkic, J., Knezevic, A., Beljic‐Ivanovic, K., Pljesa‐Ercegovac, M., Miletic, M., Soldatovic, I., Radosavljevic, T., Jovanovic, T., Simic, T., Ivanovic, V., Milasin, J.
• Format: Article
• Language: English
• Subjects: 8-Hydroxydeoxyguanosine; Adult; apical periodontitis; Biomarkers; Biomarkers - metabolism; Bone resorption; Bone Resorption - metabolism; Bone Resorption - virology; Case-Control Studies; Dentistry; Deoxyguanosine - analogs & derivatives; Deoxyguanosine - metabolism; Endodontics; Enzyme-Linked Immunosorbent Assay; Epstein-Barr virus; Epstein-Barr Virus Infections - metabolism; Female; Gene expression; Glutathione; Glutathione - metabolism; Gum disease; Herpesvirus 4, Human; Humans; Male; Molars; Osteoprotegerin; Osteoprotegerin - metabolism; Oxidative Stress; Periapical Periodontitis - metabolism; Periapical Periodontitis - virology; Periodontitis; Polymerase chain reaction; RANK ligand; RANK Ligand - metabolism; Real-Time Polymerase Chain Reaction; RNA-directed DNA polymerase; Statistical analysis; Teeth; TRANCE protein
• ISSN: 0143-2885; 1365-2591
• DOI: 10.1111/iej.12886

Aim To investigate whether apical periodontitis lesions infected by Epstein–Barr virus (EBV) exhibit higher levels of oxidative stress biomarkers [8‐hydroxydeoxyguanosine (8‐OHdG) and oxidized glutathione (GSSG)] and bone resorption regulators [receptor activator of nuclear factor (NF‐κB) ligand (RANKL) and osteoprotegerin (OPG)] compared to EBV‐negative periapical lesions and healthy pulp tissues. Methodology The experimental group consisted of 30 EBV‐positive and 30 EBV‐negative periapical lesions collected in conjunction with apicoectomy. The pulp tissues of 20 impacted third molars were used as healthy controls. The qualitative and quantitative analysis of EBV was performed by nested and real‐time polymerase chain reaction (PCR), respectively. The levels of RANKL and OPG were analysed by reverse transcriptase real‐time PCR. The levels of 8‐OHdG and GSSG were determined by enzyme‐linked immunosorbent assay (ELISA). Mann–Whitney U‐test and Spearman's correlation were used for statistical analysis. Results The levels of RANKL, OPG, 8‐OHdG and GSSG were significantly higher in apical periodontitis lesions compared to healthy pulp controls (P = 0.001, P