Bovine Serum Albumin (BSA) coated iron oxide magnetic nanoparticles as biocompatible carriers for curcumin-anticancer drug

• Published in: Bioorganic chemistry 2018-02, Vol.76, p.501-509
• Main Authors: Nosrati, Hamed, Sefidi, Naser, Sharafi, Ali, Danafar, Hossein, Kheiri Manjili, Hamidreza
• Format: Article
• Language: English
• Subjects: Albumin nanoparticles; Animals; Antineoplastic Agents - chemistry; Antineoplastic Agents - pharmacology; Bovine Serum Albumin (BSA); BSA coated Fe3O4; Cattle; Curcumin; Curcumin - chemistry; Curcumin - pharmacology; Drug Carriers - chemical synthesis; Drug Carriers - chemistry; Drug Carriers - toxicity; Drug delivery; Drug Liberation; Humans; Magnetic nanoparticles; Magnetic Phenomena; Magnetite Nanoparticles - chemistry; Magnetite Nanoparticles - toxicity; MCF-7 Cells; Particle Size; Serum Albumin, Bovine - chemistry; Serum Albumin, Bovine - toxicity
• ISSN: 0045-2068; 1090-2120
• DOI: 10.1016/j.bioorg.2017.12.033

[Display omitted] •The F@BSA NPs were prepared through desolvation and chemical coprecipitation process.•The F@BSA@CUR NPs were spherical in shape with an average size of 56 ± 11.43 nm.•F@BSA@CUR NPs had much higher cytotoxicity against MCF7 cells. The bovine serum albumin-coated magnetic nanoparticles (F@BSA NPs) were prepared as curcumin (CUR) carriers through desolvation and chemical co-precipitation process. The characteristics of CUR loaded F@BSA NPs (F@BSA@CUR NPs) were determined by X-ray diffraction (XRD), thermogravimetric analysis (TGA), fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and vibrating-sampling magnetometry (VSM) techniques. It was found that the synthesized F@BSA@CUR NPs were spherical in shape with an average size of 56 ± 11.43 nm (mean ± SD (n = 33)), ζ-potential of −10.1 mV, and good magnetic responsivity. Meanwhile, the drug content of the nanoparticles was 6.88%. These F@BSA@CUR NPs also demonstrated sustained release of CUR at 37 °C in different buffer solutions. Cellular toxicity of F@BSA@CUR NPs was studied on HFF2 cell line. Also, the cytotoxicity of F@BSA@CUR NPs towards MCF-7 breast cancer cells was investigated. The results revealed that F@BSA@CUR NPs have significant cytotoxicity activity on MCF-7 cell line.