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The A/ENTH Domain-Containing Protein AtECA4 Is an Adaptor Protein Involved in Cargo Recycling from the trans-Golgi Network/Early Endosome to the Plasma Membrane
Endocytosis and subsequent trafficking pathways are crucial for regulating the activity of plasma membrane-localized proteins. Depending on cellular and physiological conditions, the internalized cargoes are sorted at (and transported from) the trans-Golgi network/early endosome (TGN/EE) to the vacu...
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Published in: | Molecular plant 2018-04, Vol.11 (4), p.568-583 |
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description | Endocytosis and subsequent trafficking pathways are crucial for regulating the activity of plasma membrane-localized proteins. Depending on cellular and physiological conditions, the internalized cargoes are sorted at (and transported from) the trans-Golgi network/early endosome (TGN/EE) to the vacuole for degradation or recycled back to the plasma membrane. How this occurs at the molecular level remains largely elusive. Here, we provide evidence that the ENTH domain-containing protein AtECA4 plays a crucial role in recycling cargoes from the TGN/EE to the plasma membrane in Arabidopsis thaliana. AtECA4:sGFP primarily localized to the TGN/EE and plasma membrane (at low levels). Upon NaCl or mannitol treatment, AtECA4:sGFP accumulated at the TGN/EE at an early time point but was released from the TGN/EE to the cytosol at later time points. The ateca4 mutant showed higher resistance to osmotic stress and more sensitive to exogenous abscisic acid (ABA) than the wild type, as well as increased expression of ABA-inducible genes RD29A and RD29B. Consistently, ABCG25, a plasma membrane-localized ABA exporter, accumulated at the prevacuolar compartment in ateca4, indicating a defect in recycling to the plasma membrane. However, the role of AtECA4 in cargo recycling is not specific to ABCG25, as it also functions in the recycling of BRI1. These results suggest that AtECA4 plays a crucial role in the recycling of endocytosed cargoes from the TGN/EE to the plasma membrane.
AtECA4 primarily localizes to the TGN/EE and plays a role in recycling of proteins from the TGN/EE to the plasma membrane. This AtECA4-mediated recycling of cargo proteins ABCG25 and BRI1 is crucial for homeostasis of cellular ABA levels and brassinolide-mediated signaling for plant growth. |
doi_str_mv | 10.1016/j.molp.2018.01.001 |
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AtECA4 primarily localizes to the TGN/EE and plays a role in recycling of proteins from the TGN/EE to the plasma membrane. This AtECA4-mediated recycling of cargo proteins ABCG25 and BRI1 is crucial for homeostasis of cellular ABA levels and brassinolide-mediated signaling for plant growth.</description><identifier>ISSN: 1674-2052</identifier><identifier>EISSN: 1752-9867</identifier><identifier>DOI: 10.1016/j.molp.2018.01.001</identifier><identifier>PMID: 29317286</identifier><language>eng</language><publisher>England: Elsevier Inc</publisher><subject>A/ENTH ; abiotic stress ; Arabidopsis - cytology ; Arabidopsis - genetics ; Arabidopsis - metabolism ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; Calcium-Transporting ATPases - genetics ; Calcium-Transporting ATPases - metabolism ; cargo recycling ; Cell Membrane - metabolism ; Droughts ; Endosomes - metabolism ; Gene Expression Regulation, Plant ; Osmotic Pressure ; Protein Transport ; Pyridinium Compounds - metabolism ; Quaternary Ammonium Compounds - metabolism ; Salinity ; trans-Golgi Network - metabolism</subject><ispartof>Molecular plant, 2018-04, Vol.11 (4), p.568-583</ispartof><rights>2018 The Author</rights><rights>Copyright © 2018 The Author. Published by Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c466t-67ca24f1a2497618013713b3ff96b9cc147fe2e8ae188fabf67b95cde091b33e3</citedby><cites>FETCH-LOGICAL-c466t-67ca24f1a2497618013713b3ff96b9cc147fe2e8ae188fabf67b95cde091b33e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29317286$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nguyen, Hong Hanh</creatorcontrib><creatorcontrib>Lee, Myoung Hui</creatorcontrib><creatorcontrib>Song, Kyungyoung</creatorcontrib><creatorcontrib>Ahn, Gyeongik</creatorcontrib><creatorcontrib>Lee, Jihyeong</creatorcontrib><creatorcontrib>Hwang, Inhwan</creatorcontrib><title>The A/ENTH Domain-Containing Protein AtECA4 Is an Adaptor Protein Involved in Cargo Recycling from the trans-Golgi Network/Early Endosome to the Plasma Membrane</title><title>Molecular plant</title><addtitle>Mol Plant</addtitle><description>Endocytosis and subsequent trafficking pathways are crucial for regulating the activity of plasma membrane-localized proteins. Depending on cellular and physiological conditions, the internalized cargoes are sorted at (and transported from) the trans-Golgi network/early endosome (TGN/EE) to the vacuole for degradation or recycled back to the plasma membrane. How this occurs at the molecular level remains largely elusive. Here, we provide evidence that the ENTH domain-containing protein AtECA4 plays a crucial role in recycling cargoes from the TGN/EE to the plasma membrane in Arabidopsis thaliana. AtECA4:sGFP primarily localized to the TGN/EE and plasma membrane (at low levels). Upon NaCl or mannitol treatment, AtECA4:sGFP accumulated at the TGN/EE at an early time point but was released from the TGN/EE to the cytosol at later time points. The ateca4 mutant showed higher resistance to osmotic stress and more sensitive to exogenous abscisic acid (ABA) than the wild type, as well as increased expression of ABA-inducible genes RD29A and RD29B. Consistently, ABCG25, a plasma membrane-localized ABA exporter, accumulated at the prevacuolar compartment in ateca4, indicating a defect in recycling to the plasma membrane. However, the role of AtECA4 in cargo recycling is not specific to ABCG25, as it also functions in the recycling of BRI1. These results suggest that AtECA4 plays a crucial role in the recycling of endocytosed cargoes from the TGN/EE to the plasma membrane.
AtECA4 primarily localizes to the TGN/EE and plays a role in recycling of proteins from the TGN/EE to the plasma membrane. This AtECA4-mediated recycling of cargo proteins ABCG25 and BRI1 is crucial for homeostasis of cellular ABA levels and brassinolide-mediated signaling for plant growth.</description><subject>A/ENTH</subject><subject>abiotic stress</subject><subject>Arabidopsis - cytology</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Calcium-Transporting ATPases - genetics</subject><subject>Calcium-Transporting ATPases - metabolism</subject><subject>cargo recycling</subject><subject>Cell Membrane - metabolism</subject><subject>Droughts</subject><subject>Endosomes - metabolism</subject><subject>Gene Expression Regulation, Plant</subject><subject>Osmotic Pressure</subject><subject>Protein Transport</subject><subject>Pyridinium Compounds - metabolism</subject><subject>Quaternary Ammonium Compounds - metabolism</subject><subject>Salinity</subject><subject>trans-Golgi Network - metabolism</subject><issn>1674-2052</issn><issn>1752-9867</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp9kU1u2zAQhYWiQfPTXqCLgstuJHMkmZSAbgxVSQwkaVC4a4KiRi5dSnRJ2oVvk6OGrtMsu-EMwe89zuAlyUegGVBgs002WrPNcgpVRiGjFN4kF8DneVpXjL-NPeNlmtN5fp5cer-hlNGKFe-S87wugOcVu0ieVj-RLGbtw-qWfLWj1FPa2CnEqqc1eXQ2oJ7IIrTNoiRLT2S89HIbrHt9XE57a_bYk9g30q0t-Y7qoMzRYHB2JCF-EZycfHpjzVqTBwx_rPs1a6UzB9JOvfV2jIj9Sz4a6UdJ7nHsogbfJ2eDNB4_vNSr5Md1u2pu07tvN8tmcZeqkrGQMq5kXg4Qj5ozqCgUHIquGIaadbVSUPIBc6wkQlUNshsY7-q56pHW0BUFFlfJ55Pv1tnfO_RBjNorNCbOYHdeQF3Vcw6UlxHNT6hy1nuHg9g6PUp3EEDFMRmxEcdkxDEZQUHEZKLo04v_rhuxf5X8iyICX04Axi33Gp3wSuOksNcOVRC91f_zfwZ9gqAP</recordid><startdate>20180402</startdate><enddate>20180402</enddate><creator>Nguyen, Hong Hanh</creator><creator>Lee, Myoung Hui</creator><creator>Song, Kyungyoung</creator><creator>Ahn, Gyeongik</creator><creator>Lee, Jihyeong</creator><creator>Hwang, Inhwan</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20180402</creationdate><title>The A/ENTH Domain-Containing Protein AtECA4 Is an Adaptor Protein Involved in Cargo Recycling from the trans-Golgi Network/Early Endosome to the Plasma Membrane</title><author>Nguyen, Hong Hanh ; Lee, Myoung Hui ; Song, Kyungyoung ; Ahn, Gyeongik ; Lee, Jihyeong ; Hwang, Inhwan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c466t-67ca24f1a2497618013713b3ff96b9cc147fe2e8ae188fabf67b95cde091b33e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>A/ENTH</topic><topic>abiotic stress</topic><topic>Arabidopsis - cytology</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - metabolism</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Calcium-Transporting ATPases - genetics</topic><topic>Calcium-Transporting ATPases - metabolism</topic><topic>cargo recycling</topic><topic>Cell Membrane - metabolism</topic><topic>Droughts</topic><topic>Endosomes - metabolism</topic><topic>Gene Expression Regulation, Plant</topic><topic>Osmotic Pressure</topic><topic>Protein Transport</topic><topic>Pyridinium Compounds - metabolism</topic><topic>Quaternary Ammonium Compounds - metabolism</topic><topic>Salinity</topic><topic>trans-Golgi Network - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nguyen, Hong Hanh</creatorcontrib><creatorcontrib>Lee, Myoung Hui</creatorcontrib><creatorcontrib>Song, Kyungyoung</creatorcontrib><creatorcontrib>Ahn, Gyeongik</creatorcontrib><creatorcontrib>Lee, Jihyeong</creatorcontrib><creatorcontrib>Hwang, Inhwan</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular plant</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nguyen, Hong Hanh</au><au>Lee, Myoung Hui</au><au>Song, Kyungyoung</au><au>Ahn, Gyeongik</au><au>Lee, Jihyeong</au><au>Hwang, Inhwan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The A/ENTH Domain-Containing Protein AtECA4 Is an Adaptor Protein Involved in Cargo Recycling from the trans-Golgi Network/Early Endosome to the Plasma Membrane</atitle><jtitle>Molecular plant</jtitle><addtitle>Mol Plant</addtitle><date>2018-04-02</date><risdate>2018</risdate><volume>11</volume><issue>4</issue><spage>568</spage><epage>583</epage><pages>568-583</pages><issn>1674-2052</issn><eissn>1752-9867</eissn><abstract>Endocytosis and subsequent trafficking pathways are crucial for regulating the activity of plasma membrane-localized proteins. Depending on cellular and physiological conditions, the internalized cargoes are sorted at (and transported from) the trans-Golgi network/early endosome (TGN/EE) to the vacuole for degradation or recycled back to the plasma membrane. How this occurs at the molecular level remains largely elusive. Here, we provide evidence that the ENTH domain-containing protein AtECA4 plays a crucial role in recycling cargoes from the TGN/EE to the plasma membrane in Arabidopsis thaliana. AtECA4:sGFP primarily localized to the TGN/EE and plasma membrane (at low levels). Upon NaCl or mannitol treatment, AtECA4:sGFP accumulated at the TGN/EE at an early time point but was released from the TGN/EE to the cytosol at later time points. The ateca4 mutant showed higher resistance to osmotic stress and more sensitive to exogenous abscisic acid (ABA) than the wild type, as well as increased expression of ABA-inducible genes RD29A and RD29B. Consistently, ABCG25, a plasma membrane-localized ABA exporter, accumulated at the prevacuolar compartment in ateca4, indicating a defect in recycling to the plasma membrane. However, the role of AtECA4 in cargo recycling is not specific to ABCG25, as it also functions in the recycling of BRI1. These results suggest that AtECA4 plays a crucial role in the recycling of endocytosed cargoes from the TGN/EE to the plasma membrane.
AtECA4 primarily localizes to the TGN/EE and plays a role in recycling of proteins from the TGN/EE to the plasma membrane. This AtECA4-mediated recycling of cargo proteins ABCG25 and BRI1 is crucial for homeostasis of cellular ABA levels and brassinolide-mediated signaling for plant growth.</abstract><cop>England</cop><pub>Elsevier Inc</pub><pmid>29317286</pmid><doi>10.1016/j.molp.2018.01.001</doi><tpages>16</tpages><oa>free_for_read</oa></addata></record> |
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subjects | A/ENTH abiotic stress Arabidopsis - cytology Arabidopsis - genetics Arabidopsis - metabolism Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Calcium-Transporting ATPases - genetics Calcium-Transporting ATPases - metabolism cargo recycling Cell Membrane - metabolism Droughts Endosomes - metabolism Gene Expression Regulation, Plant Osmotic Pressure Protein Transport Pyridinium Compounds - metabolism Quaternary Ammonium Compounds - metabolism Salinity trans-Golgi Network - metabolism |
title | The A/ENTH Domain-Containing Protein AtECA4 Is an Adaptor Protein Involved in Cargo Recycling from the trans-Golgi Network/Early Endosome to the Plasma Membrane |
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