Nutritionally enriched 1,3-diacylglycerol-rich oil: Low calorie fat with hypolipidemic effects in rats

• Published in: Food chemistry 2018-05, Vol.248, p.210-216
• Main Authors: Prabhavathi Devi, B.L.A., Gangadhar, K.N., Prasad, R.B.N., Sugasini, D., Rao, Y. Poorna Chandra, Lokesh, B.R.
• Format: Article
• Language: English
• Subjects: Calorific value; Diacylglycerol-rich oil; Rice bran oil; Serum and liver lipids; Sunflower oil
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2017.12.066

•Enzymatic process for nutritionally enriched DAG-rich oil from a blend of SFO and RBO.•The DAG-rich oil consists of 64% of 1,3-DAG, 18% of 1,2-DAG and 16% of TAG along with micronutrients.•DAG-rich oil exhibited 30% lower calorific value as compared to SFO.•DAG-rich oil reduced LDL cholesterol, triglyceride levels in serum and liver of rats as compared to SFO.•DAG-rich oil can function as low calorie fat as well as well as hypolipidemic agent. An enzymatic process was developed for the preparation of a nutritionally enriched 1,3-diacylglycerol(DAG)-rich oil from a blend of refined sunflower and rice bran oils. The process involves hydrolysis of vegetable oil blend using Candida cylindracea followed by esterification with glycerol using Lipozyme RM1M. The resultant DAG-rich oil contains 84% of DAG (66% of 1,3-DAG, 18% of 1,2-DAG) and 16% of triacylglycerol (TAG) along with micro nutrients like γ-oryzanol, tocotrienols, tocopherols and phytosterols. Nutritional studies of the DAG-rich oil were conducted in Wistar rats and compared with sunflower oil (SFO). The calorific value of the DAG-rich oil was estimated to be 6.45 Kcals/g as against 9.25 Kcals/g for SFO. The serum and liver cholesterol and TAG levels in rats fed with 1,3-DAG-rich oil were found to be significantly reduced as compared to rats fed diet containing SFO. We conclude that 1,3-DAG-rich oil is a low calorie fat and exhibits hypolipidemic effects.