Structural Basis of Heterochromatin Formation by Human HP1

• Published in: Molecular cell 2018-02, Vol.69 (3), p.385-397.e8
• Main Authors: Machida, Shinichi, Takizawa, Yoshimasa, Ishimaru, Masakazu, Sugita, Yukihiko, Sekine, Satoshi, Nakayama, Jun-ichi, Wolf, Matthias, Kurumizaka, Hitoshi
• Format: Article
• Language: English
• Subjects: ACF; chromatin; Chromatin - metabolism; Chromatin Assembly and Disassembly - genetics; Chromosomal Proteins, Non-Histone - genetics; Chromosomal Proteins, Non-Histone - metabolism; cryo-EM; Cryoelectron Microscopy - methods; DNA - metabolism; epigenetics; H3K9me3; heterochromatin; Heterochromatin - metabolism; histone; Histones - metabolism; HP1; Humans; Jumonji Domain-Containing Histone Demethylases - metabolism; Methylation; nucleosome; Nucleosomes - metabolism; Protein Binding; Structure-Activity Relationship; Transcription Factors - metabolism
• ISSN: 1097-2765; 1097-4164
• DOI: 10.1016/j.molcel.2017.12.011

Heterochromatin plays important roles in transcriptional silencing and genome maintenance by the formation of condensed chromatin structures, which determine the epigenetic status of eukaryotic cells. The trimethylation of histone H3 lysine 9 (H3K9me3), a target of heterochromatin protein 1 (HP1), is a hallmark of heterochromatin formation. However, the mechanism by which HP1 folds chromatin-containing H3K9me3 into a higher-order structure has not been elucidated. Here we report the three-dimensional structure of the H3K9me3-containing dinucleosomes complexed with human HP1α, HP1β, and HP1γ, determined by cryogenic electron microscopy with a Volta phase plate. In the structures, two H3K9me3 nucleosomes are bridged by a symmetric HP1 dimer. Surprisingly, the linker DNA between the nucleosomes does not directly interact with HP1, thus allowing nucleosome remodeling by the ATP-utilizing chromatin assembly and remodeling factor (ACF). The structure depicts the fundamental architecture of heterochromatin. [Display omitted] •The HP1-H3K9me3 dinucleosome complex structure determined by the cryo-EM method•HP1 forms a symmetric dimer and bridges two H3K9me3 nucleosomes in the complex•The HP1 chromoshadow domain dimer exists in an accessible location in the complex•The linker DNA between nucleosomes does not directly interact with HP1 HP1 and H3 Lys9 trimethylations (H3K9me3) are hallmarks of heterochromatin, and they play pivotal roles in the epigenetic propagation of heterochromatin. Machida et al. describe the structure of the H3K9me3-containing dinucleosome complexed with human HP1, obtained by the cryo-EM technique.