Biofuel production and phycoremediation by Chlorella sp. ISTLA1 isolated from landfill site

• Published in: Bioresource technology 2018-04, Vol.253, p.121-129
• Main Authors: Mishra, Arti, Medhi, Kristina, Maheshwari, Neha, Srivastava, Shaili, Thakur, Indu Shekhar
• Format: Article
• Language: English
• Subjects: Bioremediation; Chlorella; Fatty acid methyl esters (FAMEs); Heavy metals adsorption; Response surface methodology (RSM)
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2017.12.012

[Display omitted] •Chlorella sp. ISTLA1 shows optimal growth in pH 9 and 100 mM NaHCO3.•FAME profile shows dominance of palmitic acid (C16:0) and oleic acid (C18:1).•Using RSM, the yield of lipid and biomass was 365.42 and 833.14 mg L−1 respectively.•It could effectually remediate heavy metals like Zn, Cu, Fe and Mn from wastewater. The present study aims to investigate the biofuel production ability and potential of heavy metal remediation of Chlorella sp. ISTLA1 isolated from a landfill site. The strain was cultured in Bold’s Basal medium at different concentration of NaHCO3 and pH. Response surface methodology was employed for the optimization of nutrient sources for higher lipid production. Under the optimized conditions, the yield of lipid and biomass was 365.42 and 833.14 mg L−1 respectively. GC–MS analysis of lipid indicated the presence of C8 to C31 organic compounds consisting mainly of palmitic acid (C16:0), stearic acid (C18:0) and oleic acid (C18:1). Additionally, remediation of heavy metals like Zn, Cu, Mn and Fe from waste water was observed by AAS and EDX. The removal efficiency was 82.6% for Zn, 56.5% for Cu, 79.8% for Mn and 40% for Fe. The study revealed simultaneous biodiesel production and waste water treatment by Chlorella sp. ISTLA1.