Metabolic alterations in a rat model of hepatic ischaemia reperfusion injury: In vivo hyperpolarized 13 C MRS and metabolic imaging

Despite a number of studies addressing the pathophysiology of hepatic IRI, a gold standard test for early diagnosis and evaluation of IRI remains elusive. This study investigated the metabolic alterations in a rat model of hepatic IRI using the in vivo hyperpolarized ¹³C MRS and metabolic imaging. H...

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Published in:Liver international 2018-06, Vol.38 (6), p.1117-1127
Main Authors: Moon, Chung-Man, Shin, Sang-Soo, Lim, Nam-Yeol, Kim, Seul-Kee, Kang, Yang-Joon, Kim, Hyoung-Ook, Lee, Seung-Jin, Beak, Byung-Hyun, Kim, Yun-Heon, Jeong, Gwang-Woo
Format: Article
Language:English
Subjects:
Animals
Body Weight
Carbon Isotopes - pharmacokinetics
Diffusion Magnetic Resonance Imaging
Disease Models, Animal
Lactic Acid - pharmacokinetics
Liver - pathology
Liver Diseases - diagnostic imaging
Liver Diseases - metabolism
Male
Rats
Rats, Sprague-Dawley
Reperfusion Injury - diagnostic imaging
Reperfusion Injury - metabolism
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Summary:Despite a number of studies addressing the pathophysiology of hepatic IRI, a gold standard test for early diagnosis and evaluation of IRI remains elusive. This study investigated the metabolic alterations in a rat model of hepatic IRI using the in vivo hyperpolarized ¹³C MRS and metabolic imaging. Hyperpolarized C MRS with IVIM-DWI was performed on the liver of 7 sham-operated control rats and 7 rats before and after hepatic IRI. The hepatic IRI-induced rats showed significantly higher ratios of [1- C] alanine/pyruvate, [1- C] alanine/tC, [1- C] lactate/pyruvate and [1- C] lactate/tC compared with both sham-operated controls and rats before IRI, whereas [1- C] pyruvate/tC ratio was decreased in IRI-induced rats. In IVIM-DWI study, apparent diffusion coefficient (ADC), f and D values in rats after hepatic IRI were significantly lower than those of rats before IRI and sham-operated controls. The levels of [1- C] alanine and [1- C] lactate were negatively correlated with ADC, f and D values, whereas the level of [1- C] pyruvate was positively correlated with these values. The levels of [1- C] alanine, [1- C] lactate and [1- C] pyruvate in conjunction with IVIM-DWI will be helpful to evaluate the hepatic IRI as well as these findings can be useful in understanding the biochemical mechanism associated with hepatic damage.
ISSN:1478-3223
1478-3231
DOI:10.1111/liv.13695