A recovery time after warming restores mitochondrial function and improves developmental competence of vitrified ovine oocytes

The aim of the present study was to assess the ability of vitrified/warmed oocyte to recover from vitrification-induced damages after warming. In vitro matured, vitrified/warmed ovine oocytes were assessed for developmental competence, mitochondrial activity and distribution, ATP, ROS and catalase l...

Full description

Saved in:
Bibliographic Details
Published in:Theriogenology 2018-04, Vol.110, p.18-26
Main Authors: Succu, Sara, Gadau, Sergio Domenico, Serra, Elisa, Zinellu, Angelo, Carru, Ciriaco, Porcu, Cristian, Naitana, Salvatore, Berlinguer, Fiammetta, Leoni, Giovanni Giuseppe
Format: Article
Language:English
Subjects:
Animals
Blastocyst yield
Cells, Cultured
Cryopreservation - veterinary
Embryo Culture Techniques
Embryonic Development - physiology
Energetic status
Female
Fertilization in Vitro - veterinary
Hot Temperature
In Vitro Oocyte Maturation Techniques - veterinary
Male
Mitochondria - physiology
Oocyte
Oocytes
Oogenesis - physiology
Recovery of Function - physiology
Restoration
Sheep - physiology
Vitrification
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The aim of the present study was to assess the ability of vitrified/warmed oocyte to recover from vitrification-induced damages after warming. In vitro matured, vitrified/warmed ovine oocytes were assessed for developmental competence, mitochondrial activity and distribution, ATP, ROS and catalase levels during 6 h of in vitro culture using fresh oocytes as control. ATP content in vitrified oocytes was lower than control during 4 h of post warming culture (p 
ISSN:0093-691X
1879-3231
DOI:10.1016/j.theriogenology.2017.12.031