Effects of macrophage migration inhibitory factor on cardiac reperfusion injury in mice with depression induced by constant-darkness

• Published in: Journal of affective disorders 2018-03, Vol.229, p.403-409
• Main Authors: Tao, Lu-yuan, Huang, Ming-yuan, Saroj-Thapa, Wang, Jiao-ni, Wu, Shao-ze, He, Fei, Huang, Kai-yu, Xue, Yang-jing, Lingwei-Jin, Liao, Lian-Ming, Tang, Ji-fei, Ji, Kang-ting
• Format: Article
• Language: English
• Subjects: AMPK; Dark environment; Depression; MIF; Myocardial ischemia-reperfusion injury
• ISSN: 0165-0327; 1573-2517
• DOI: 10.1016/j.jad.2017.12.039

Depression is associated with coronary artery disease and increases adverse outcomes and mortality in patients with acute myocardial infarction, but the underlying pathophysiological mechanisms remain unclear. To evaluate the effect of macrophage migration inhibitory factor (MIF) on cardiac ischemia-reperfusion (I/R) injury in mice with constant darkness-induced depression. Twenty C57BL/6 mice (8 weeks old, male) were randomly divided into 2 groups: one group was housed in a 12h light/dark cycle environment (LD) and the other in a constant darkness environment (DD). After 3 weeks, constant darkness-exposed (DD) mice displayed depression-like behavior as indicated by increased immobility in the forced swim test (FST) and lower sucrose preference rate. Western blotting revealed cardiac MIF expression was significantly lower in the DD mice than that in the LD mice. Next, 84 mice were randomly divided into 4 groups: LD sham group, LD I/R group, DD sham group, and DD I/R group. Following ischemia and reperfusion, mice in the DD I/R group had a larger infarct area and lower heart function index than mice in the LD I/R group (P < 0.05 for both). The cardiac pAMPK and pACC expression levels of the DD I/R group were also lower in the DD I/R group (P < 0.05). DD-induced depression might cause decreased expression of MIF in the heart, resulting in downregulation of MIF-AMPK signaling and a subsequent adverse outcome after a cardiac I/R injury. •Our animal experiments show that an environment with long periods of exposure to constant darkness can lead to depression-like symptoms in mice.•We used this model to investigate how depression aggravates mice cardiac reperfusion injury.•We demonstrate that MIF (macrophage migration inhibitory factor), an inflammatory factor that plays an important role in energy metabolism under ischemia reperfusion injury, may prevent cardiac ischemia reperfusion injury in mice with depression.•To the best of our knowledge, this is the first time that MIF is found to be involved in the worsening of the cardiac I/R injury in depressive mice.