Effects of intravenous tryptophan infusion on thermoregulation in steers exposed to acute heat stress

• Published in: Animal science journal 2018-05, Vol.89 (5), p.777-783
• Main Authors: Sutoh, Madoka, Kasuya, Etsuko, Yayou, Ken‐ichi
• Format: Article
• Language: English
• Subjects: Animals; Body Temperature - physiology; Body Temperature Regulation - drug effects; Bovidae; Brain; Brain - metabolism; Cattle; Cattle - metabolism; Cattle - physiology; Cerebrospinal fluid; Heat; Heat loss; Heat stress; Heat tolerance; Hot Temperature - adverse effects; Infusions, Intravenous; Intravenous administration; Male; Metabolites; Peripheral blood; Prolactin; Rectum - physiology; Serotonin; Serotonin - cerebrospinal fluid; Skin; Skin Physiological Phenomena; Stress, Physiological - physiology; Temperature effects; Thermoregulation; Thyroid hormones; Triiodothyronine; Tryptophan; Tryptophan - administration & dosage; Tryptophan - pharmacology
• ISSN: 1344-3941; 1740-0929
• DOI: 10.1111/asj.12988

This study was conducted to investigate the effect of tryptophan (TRP) supply on the thermoregulatory responses via brain serotonin (5‐HT) in cattle. In period 1, 12 Holstein steers were kept under a constant room temperature (22°C) and were administered the intravenous (i.v.) infusion of saline or TRP (38.5 mg/kg/2 h). Changes in rectal temperature (RT), 5‐HT concentration in the cerebrospinal fluid (CSF), and other factors involved in thermoregulation were measured. In period 2, the steers received the same treatments as in period 1; however, the room temperature was elevated from 22°C to 33°C during i.v. infusion and maintained at 33°C for 3 h. 5‐HT concentration in CSF increased following TRP infusion in both periods, and RT significantly decreased following TRP infusion only in period 2. The effect of TRP on respiration rate and plasma prolactin and total triiodothyronine concentrations was not significant. These results suggest that increase in TRP supply can attenuate increase in RT in response to acute heat stress through the increase in brain 5‐HT, followed by presumable increase in evaporative heat loss from the skin surface in cattle. It is possible that the increase in peripheral blood TRP metabolites could also participate in the hypothermic effect of TRP.