Interspecific hybrids between the homothallic Phytophthora sojae and Phytophthora vignae

An interspecific cross was attempted between two homothallic species of Phytophthora, P. sojae and P. vignae. From 1640 single-oospore cultures isolated, DNA was extracted from 800, and two interspecific F^sub 1^ hybrids (F^sub 1^ 1121 and F^sub 1^ 1426) were putatively identified using RAPD markers...

Full description

Saved in:
Bibliographic Details
Published in:Australasian plant pathology 2003-01, Vol.32 (3), p.353-359
Main Authors: May, K J, Drenth, A, Irwin, JAG
Format: Article
Language:English
Subjects:
Amplified fragment length polymorphism
DNA
Host specificity
Interspecific hybridization
Oomycetes
Pathogenicity
Phytophthora
Phytophthora sojae
Random amplified polymorphic DNA
Seedlings
Soybeans
Virulence
Wilting
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:An interspecific cross was attempted between two homothallic species of Phytophthora, P. sojae and P. vignae. From 1640 single-oospore cultures isolated, DNA was extracted from 800, and two interspecific F^sub 1^ hybrids (F^sub 1^ 1121 and F^sub 1^ 1426) were putatively identified using RAPD markers. The true hybrid nature of these F^sub 1^ hybrids was confirmed using additional AFLP analysis. Single-zoospore cultures were generated for each F^sub 1^ hybrid and one single-zoospore culture of each was used in pathogenicity and virulence tests. Both F^sub 1^ hybrids were pathogenic to soybean and cowpea, causing symptoms including lesions, wilting and death of susceptible soybean and cowpea cultivars. However, the aggressiveness of the F^sub 1^ hybrids was reduced and was substantially more variable when compared with that of the parental isolates on their respective hosts. The F^sub 1^ hybrids were reisolated from infected seedlings and their hybrid nature confirmed using RAPD and AFLP analysis. These results provide a basis for further research aimed at obtaining an increased understanding of the genetics of host specificity in the Oomycetes.[PUBLICATION ABSTRACT]
ISSN:0815-3191
1448-6032
DOI:10.1071/AP03037