Eleven new microsatellite markers for the tropical sea urchin Tripneustes gratilla and cross-amplification in Tripneustes ventricosa

We describe the isolation, characterization, and application of 11 polymorphic microsatellite loci from the tropical sea urchin Tripneustes gratilla. We genotyped a total of 73 individuals in three subpopulations (two in Hawaii, and one in the Philippines) and found between eight and 33 alleles per...

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Bibliographic Details
Published in:Molecular ecology notes 2007-11, Vol.7 (6), p.1002-1004
Main Authors: CARLON, DAVID B., LIPPÉ, CATHERINE
Format: Article
Language:English
Subjects:
coral reefs
Echinoidea
herbivores
Marine
null alleles
population structure
Tripneustes
Tripneustes gratilla
Tripneustes ventricosus
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Summary:We describe the isolation, characterization, and application of 11 polymorphic microsatellite loci from the tropical sea urchin Tripneustes gratilla. We genotyped a total of 73 individuals in three subpopulations (two in Hawaii, and one in the Philippines) and found between eight and 33 alleles per locus. We found significant heterozygote deficits within at least one subpopulation at seven out of 11 loci. We were successful at cross‐amplifying four loci in the congeneric Tripneustes ventricosus. When used appropriately, this set of loci will be useful for quantifying population structure in Tripneustes urchins across broad geographical ranges.
ISSN:1471-8278
1471-8286
DOI:10.1111/j.1471-8286.2007.01755.x