Synthesis of a novel HER2 targeted aza-BODIPY-antibody conjugate: synthesis, photophysical characterisation and in vitro evaluation

We herein report the synthesis and analysis of a novel aza-BODIPY-antibody conjugate, formed by controlled and regioselective bioconjugation methodology. Employing the clinically relevant antibody, which targets HER2 positive cancers, represents an excellent example of an antibody targeting strategy...

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Bibliographic Details
Published in:Organic & biomolecular chemistry 2018, Vol.16 (7), p.1144-1149
Main Authors: Cheng, Miffy H Y, Maruani, Antoine, Savoie, Huguette, Chudasama, Vijay, Boyle, Ross W
Format: Article
Language:English
Subjects:
Antibodies - chemistry
Antibodies - immunology
Boron Compounds - chemistry
Boron Compounds - immunology
Breast Neoplasms - diagnostic imaging
ErbB-2 protein
Female
Fluorescence
Humans
Immunoconjugates - chemistry
Infrared Rays
Neoplasm Proteins - analysis
Photochemical Processes
Protein Binding
Receptor, ErbB-2 - analysis
Receptor, ErbB-2 - immunology
Synthesis
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Summary:We herein report the synthesis and analysis of a novel aza-BODIPY-antibody conjugate, formed by controlled and regioselective bioconjugation methodology. Employing the clinically relevant antibody, which targets HER2 positive cancers, represents an excellent example of an antibody targeting strategy for this class of near-IR emitting fluorophore. The NIR fluorescence and binding properties were validated through in vitro studies using live cell confocal imaging.
ISSN:1477-0520
1477-0539
DOI:10.1039/c7ob02957h