Enzymatic syntheses of dopamine glycosides

Glucoamylase from Rhizopus mold and β-glucosidase catalysed synthesis of dopamine- d-glucoside was optimized in terms of pH, buffer, enzyme, dopamine concentrations and incubation period in organic media. Under the optimum conditions, β-glucosidase showed regioselectivity by giving rise to 3-hydroxy...

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Bibliographic Details
Published in:Enzyme and microbial technology 2009-01, Vol.44 (1), p.33-39
Main Authors: Sivakumar, Ramaiah, Divakar, Soundar
Format: Article
Language:English
Subjects:
ACE inhibition
Antioxidant activity
Bioconversions. Hemisynthesis
Biological and medical sciences
Biotechnology
Dopamine glycosides
Enzymatic synthesis
Fundamental and applied biological sciences. Psychology
Glucoamylase
Methods. Procedures. Technologies
Rhizopus
β-Glucosidase
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Summary:Glucoamylase from Rhizopus mold and β-glucosidase catalysed synthesis of dopamine- d-glucoside was optimized in terms of pH, buffer, enzyme, dopamine concentrations and incubation period in organic media. Under the optimum conditions, β-glucosidase showed regioselectivity by giving rise to 3-hydroxy-4- O-(β- d-glucopyranosyl)phenylethylamine 5b. Presence of hydroxyl group at 3rd and 4th position of phenyl ring showed only monoglycosylation/arylation products with d-glucose 2, d-galactose 3 and d-mannose 4 for both the enzymes. Synthesized glycosides also exhibited ACE inhibition and antioxidant activities.
ISSN:0141-0229
1879-0909
DOI:10.1016/j.enzmictec.2008.09.014