Flow cytometric detection of DNA cell cycle alterations in hemocytes of mussels ( Mytilus galloprovincialis) off the Adriatic coast, Croatia

Studies were carried out to determine the alteration in DNA cell cycle characteristics of hemocytes of the mussel Mytilus galloprovincialis collected at 17 different locations (146 individuals) along the Adriatic coast, Croatia. In order to connect possible genomic manifestation to urban and/or indu...

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Bibliographic Details
Published in:Aquatic toxicology 2003-07, Vol.64 (2), p.121-129
Main Authors: Bihari, Nevenka, Mičić, Milena, Batel, Renato, Zahn, Rudolf K.
Format: Article
Language:English
Subjects:
Adriatic sea
Agnatha. Pisces
Aneuploidy
Animal, plant and microbial ecology
Animals
Applied ecology
Biological and medical sciences
Bivalvia
Bivalvia - cytology
Bivalvia - drug effects
Bivalvia - genetics
Cell Cycle - drug effects
Cell Cycle - genetics
Croatia
DNA - analysis
DNA cell cycle alterations
DNA Damage
Ecotoxicology, biological effects of pollution
Effects of pollution and side effects of pesticides on protozoa and invertebrates
Effects of pollution and side effects of pesticides on vertebrates
Environmental Exposure
Flow Cytometry
Fundamental and applied biological sciences. Psychology
Hemocytes - drug effects
Industrial Waste - adverse effects
Marine
Mediterranean Sea
Mussel
Mutagens - toxicity
Mytilus galloprovincialis
Water Pollutants, Chemical - toxicity
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Summary:Studies were carried out to determine the alteration in DNA cell cycle characteristics of hemocytes of the mussel Mytilus galloprovincialis collected at 17 different locations (146 individuals) along the Adriatic coast, Croatia. In order to connect possible genomic manifestation to urban and/or industrial waste flow cytometry was used. We studied incidence of altered DNA profile reflective of chromosomal fragmentation phenomena or aneuploid mosaicism, coefficient of variation (CV) in DNA fluorescence as a measure of intraindividual genome size variability and DNA index (DI) as a measure of ploidy. The different classes of DNA cell cycle alterations found in this study mirror either acute or cumulative genotoxic effects of the surrounding environment on mussel hemocyte DNA. These are intraindividual genome size variability (CV>8, seven individuals from four sites), aneuploidy (altered DNA profile and DI
ISSN:0166-445X
1879-1514
DOI:10.1016/S0166-445X(03)00040-7