Inactivation of Legionella pneumophila from Whirlpool Bath Waters by Grapefruit (Citrus paradisi) Seed Extract

We investigated the antibacterial effect of commercial grapefruit seed extract (GSE, Liquid), which has recently been attracting attention as a food additive, on Legionella pneumophila. The sensitivity test was performed using the disc method. All 25 strains tested were sensitive to GSE, and formed...

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Bibliographic Details
Published in:Biocontrol Science 2003/09/20, Vol.8(3), pp.129-132
Main Authors: FURUHATA, KATSUNORI, DOGASAKI, CHIKAKU, HARA, MOTONOBU, FUKUYAMA, MASAFUMI
Format: Article
Language:English
Subjects:
Bactericidal activity
Citrus paradisi
Grapefruit seed extract
Legionella pneumophila
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Summary:We investigated the antibacterial effect of commercial grapefruit seed extract (GSE, Liquid), which has recently been attracting attention as a food additive, on Legionella pneumophila. The sensitivity test was performed using the disc method. All 25 strains tested were sensitive to GSE, and formed clear inhibition zones in its presence. The minimum inhibitory concentration (MIC) values were measured by the agar dilution method. The MIC of GSE in the strains was in the range of 98mg/l to 390mg/l, and the MIC90 was 390mg/l. The minimum bactericidal concentration (MBC) values for the test strains were also determined. MBC90 was 50, 000, 6, 300, and 3, 100mg/l when the exposure time was 1, 10, and 60 minutes, respectively. These bactericidal test results demonstrate that GSE has a bactericidal effect on L. pneumophila.
ISSN:1342-4815
1884-0205
DOI:10.4265/bio.8.129