In vitro inhibitory effect of gossypol from gossypol‐acetic acid, and (+)‐ and (−)‐isomers of gossypol on the growth of Edwardsiella ictaluri

• Published in: Journal of applied microbiology 2004-07, Vol.97 (1), p.87-92
• Main Authors: Yildirim‐Aksoy, M., Lim, C., Dowd, M.K., Wan, P.J., Klesius, P.H., Shoemaker, C.
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - pharmacology; bacteria; Bacteriological Techniques; Biological and medical sciences; channel catfish; Edwardsiella ictaluri; Edwardsiella ictaluri - drug effects; Edwardsiella ictaluri - growth & development; Fundamental and applied biological sciences. Psychology; gossypol; Gossypol - pharmacology; gossypol enantiomers; growth inhibitory effect; Microbial Sensitivity Tests; Microbiology
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1111/j.1365-2672.2004.02273.x

Aim:  This study was conducted to evaluate the toxic effect of gossypol from gossypol‐acetic acid, and (+)‐ and (−)‐isomers of gossypol on the growth of Edwardsiella ictaluri. Methods and Results:  Inhibitory effect of various concentrations of gossypol on the growth of E. ictaluri was determined. Bacterial recovery was performed by preincubation of bacteria in medium containing various concentrations of gossypol and subsequent activation of bacteria by inoculating on gossypol‐free plates. Concentrations of racemic gossypol, (+)‐gossypol and (−)‐gossypol of 1·5 μg ml−1 or higher significantly reduced the number of bacterial colonies compared with that of the control. The growth of E. ictaluri was completely inhibited on agar plates supplemented with 3 μg ml−1, regardless of the forms of gossypol. The inhibitory effect of (+)‐gossypol was higher than that of (−)‐gossypol or gossypol‐acetic acid. Recovery of E. ictaluri was