Singlepathr registered Salmonella: Performance-Tested Method super(SM) 060401

Singlepath registered Salmonella is an immunochromatographic (lateral flow) assay for the presumptive qualitative detection of Salmonella spp. in food. The AOAC Performance-Tested Method super(SM) study evaluated Singlepath Salmonella as an effective method for the detection of Salmonella spp. in th...

Full description

Saved in:
Bibliographic Details
Published in:Journal of AOAC International 2006-04, Vol.89 (2), p.417-432
Main Authors: Thompson, L, Lindhardt, C, Odumeru, J, Eifert, J D
Format: Article
Language:English
Subjects:
Piper nigrum
Salmonella
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Singlepath registered Salmonella is an immunochromatographic (lateral flow) assay for the presumptive qualitative detection of Salmonella spp. in food. The AOAC Performance-Tested Method super(SM) study evaluated Singlepath Salmonella as an effective method for the detection of Salmonella spp. in the following selected foods: dried skimmed milk, black pepper, dried pet food, desiccated coconut, cooked peeled frozen prawns, raw ground beef, and raw ground turkey. When the foods were inoculated with Salmonella spp. at levels ranging from low [0.23-1.08 colony forming units (CFU)/25 g] to high (2.3-6.0 CFU/25 g), a Chi-square value of 0.9 indicated that there was no significant difference between Singlepath Salmonella and the ISO 6579:2002 reference method. Singlepath Salmonella gave a false-positive rate of 7.3%and a false-negative rate of 2.5%. For the inclusivity study, all 105 Salmonella serovars reacted with Singlepath Salmonella. For the exclusivity study, 58 non-Salmonella spp. were tested. There were no cross-reactions with Singlepath Salmonella from these strains.
ISSN:1060-3271