Natural attenuation of chlorinated solvents at Area 6, Dover Air Force Base: characterization of microbial community structure

A polyphasic approach based on cultivation and direct recovery of 16S rRNA gene sequences was utilized for microbial characterization of an aquifer contaminated with chlorinated ethenes. This work was conducted in order to support the evaluation of natural attenuation of chlorinated ethenes in groun...

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Bibliographic Details
Published in:Journal of contaminant hydrology 2002-07, Vol.57 (1), p.41-59
Main Authors: Davis, John W., Odom, J.Martin, DeWeerd, Kim A., Stahl, David A., Fishbain, Susan S., West, Robert J., Klecka, Gary M., DeCarolis, John G.
Format: Article
Language:English
Subjects:
Biodegradation
Biodegradation, Environmental
Biological and medical sciences
Biological treatment of waters
Biomass
Bioremediation
Biotechnology
Chlorinated solvents
Chlorine Compounds - analysis
Chlorine Compounds - metabolism
Dehalobacter
Delaware
Desulfitobacterium
DNA, Bacterial - analysis
DNA, Bacterial - genetics
Environment and pollution
Environmental Monitoring
Freshwater
Fundamental and applied biological sciences. Psychology
Geologic Sediments - chemistry
Geologic Sediments - microbiology
Industrial applications and implications. Economical aspects
Microorganisms
Natural attenuation
Proteobacteria
Protista
RNA, Ribosomal, 16S - analysis
Solvents - analysis
Solvents - metabolism
USA, Delaware, Dover
Water Microbiology
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Summary:A polyphasic approach based on cultivation and direct recovery of 16S rRNA gene sequences was utilized for microbial characterization of an aquifer contaminated with chlorinated ethenes. This work was conducted in order to support the evaluation of natural attenuation of chlorinated ethenes in groundwater at Area 6 at Dover Air Force Base (Dover, DE). Results from these studies demonstrated the aquifer contained relatively low biomass (e.g. direct microscopic counts of
ISSN:0169-7722
1873-6009
DOI:10.1016/S0169-7722(01)00217-0