miR15a and miR16 in Chilean type 1 diabetes patients: possible association with apoptosis, inflammatory, or autoimmunity markers

Aim Type 1 diabetes mellitus (T1D) is an autoimmune disease characterized by the progressive destruction of β cells, mediated by the interaction between T cells and several cytokines. The pathogenesis of T1D has established its possible relationship with miRNAs. In this study, we analyze the express...

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Bibliographic Details
Published in:Journal of endocrinological investigation 2018-09, Vol.41 (9), p.1083-1088
Main Authors: Garcia-Diaz, D. F., Camacho-Guillén, P., Codner, E., Pérez-Bravo, F.
Format: Article
Language:English
Subjects:
Adolescent
Adult
Apoptosis
Apoptosis - drug effects
Apoptosis - physiology
Autoantibodies
Autoimmune diseases
Autoimmunity - drug effects
Autoimmunity - physiology
Biomarkers - metabolism
Cells, Cultured
Child
Child, Preschool
Chile - epidemiology
Diabetes
Diabetes mellitus
Diabetes mellitus (insulin dependent)
Diabetes Mellitus, Type 1 - epidemiology
Diabetes Mellitus, Type 1 - immunology
Diabetes Mellitus, Type 1 - metabolism
Endocrinology
Female
Humans
Inflammation
Inflammation Mediators - metabolism
Interleukin 6
Leukocytes (mononuclear)
Leukocytes, Mononuclear - drug effects
Leukocytes, Mononuclear - immunology
Leukocytes, Mononuclear - metabolism
Lymphocytes T
Male
Medicine
Medicine & Public Health
Metabolic Diseases
MicroRNAs - biosynthesis
Original Article
Peripheral blood mononuclear cells
Tumor Necrosis Factor-alpha - toxicity
Tumor necrosis factor-α
Young Adult
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Summary:Aim Type 1 diabetes mellitus (T1D) is an autoimmune disease characterized by the progressive destruction of β cells, mediated by the interaction between T cells and several cytokines. The pathogenesis of T1D has established its possible relationship with miRNAs. In this study, we analyze the expression profile of miR-15a and miR-16 in peripheral blood mononuclear cells (PBMCs) and their possible association with apoptosis, inflammation, or autoimmunity markers. Patients and methodology 38 T1D patients and 41 control subjects were recruited. mRNAs were analyzed by means of qPCR and TaqMan probes. PBMCs were treated with different concentrations of glucose (baseline, 11 and 25 mM) with or without an inflammatory stimulus as TNF-α (10 ng/ml). Results A decrease in the levels of the miR-15a expression in basal conditions is observed in T1D patients compared to healthy control subjects (relative units 0.5 vs. 1.8, p  
ISSN:1720-8386
0391-4097
1720-8386
DOI:10.1007/s40618-018-0837-9