Selenium (Na2SeO3) Upregulates Expression of Immune Genes and Blood–Testis Barrier Constituent Proteins of Bovine Sertoli Cell In Vitro

• Published in: Biological trace element research 2018-10, Vol.185 (2), p.332-343
• Main Authors: Adegoke, E.O, Wang, Xue, Wang, Hao, Wang, Chen, Zhang, Han, Zhang, Guixue
• Format: Article
• Language: English
• Subjects: Animals; Biochemistry; Biomedical and Life Sciences; Biotechnology; Blood; Blood-Testis Barrier - drug effects; Blood-Testis Barrier - metabolism; Cadherins - genetics; Cadherins - immunology; Cadherins - metabolism; Calves; Cattle; Cell culture; Cell Survival - drug effects; Cells, Cultured; Connexin 43; Connexin 43 - genetics; Connexin 43 - immunology; Connexin 43 - metabolism; Culture media; Cytokines; Cytotoxicity; DNA; Dose-Response Relationship, Drug; E-cadherin; Gene expression; Genes; Immune system; Immunity - genetics; Inflammation; Interferon; Interleukin 10; Interleukin 4; Life Sciences; Male; Nucleotide sequence; Nutrition; Occludin - genetics; Occludin - immunology; Occludin - metabolism; Oncology; PCR; Proteins; Selenium; Sertoli cells; Sertoli Cells - drug effects; Sertoli Cells - metabolism; Sodium; Sodium selenite; Sodium Selenite - pharmacology; Structure-Activity Relationship; Supplements; Tight Junctions - genetics; Tight Junctions - immunology; Tight Junctions - metabolism; TLR4 protein; Toll-like receptors; Transforming growth factor-b1; Tumor necrosis factor-α; Up-Regulation - drug effects; γ-Interferon
• ISSN: 0163-4984; 1559-0720
• DOI: 10.1007/s12011-018-1248-7

Sertoli cells were isolated from newborn calves and cultured in a medium supplemented with 0, 0.25, 0.50, 0.75, and 1.00 mg/L of sodium selenite to study their immune stimulatory effect, influence on cell’s viability, and expression of blood–testis barrier proteins (occludin, connexin-43, zonula occluden, E-cadherin) using quantitative PCR and western blot analyses. Results showed that medium supplemented with 0.50 mg/L of selenium significantly ( P