Associations of polymorphisms in the cytokine genes IL1β (rs16944), IL6 (rs1800795), IL12b (rs3212227) and growth factor VEGFA (rs2010963) with anthracosilicosis in coal miners in Russia and related genotoxic effects

• Published in: Mutagenesis 2018-04, Vol.33 (2), p.129-135
• Main Authors: Volobaev, Valentin P, Larionov, Aleksey V, Kalyuzhnaya, Ekaterina E, Serdyukova, Ekaterina S, Yakovleva, Svetlana, Druzhinin, Vladimir G, Babich, Olga O, Hill, Elena G, Semenihin, Victor A, Panev, Nikolay I, Minina, Varvara I, Sivanesan, Saravana Devi, Naoghare, Pravin, da Silva, Juliana, Barcelos, Gustavo R M, Prosekov, Alexander Y
• Format: Article
• Language: English
• Subjects: Adult; Anthracosilicosis - etiology; Anthracosilicosis - genetics; Anthracosilicosis - pathology; Chromosome Aberrations - drug effects; Coal - adverse effects; Coal Mining; DNA Damage - drug effects; Genetic Association Studies; Genetic Predisposition to Disease; Genotype; Humans; Interleukin-12 Subunit p40 - genetics; Interleukin-1beta - genetics; Interleukin-6 - genetics; Male; Middle Aged; Miners; Occupational Exposure; Polymorphism, Single Nucleotide - genetics; Silicon Dioxide - isolation & purification; Silicon Dioxide - toxicity; Vascular Endothelial Growth Factor A - genetics
• ISSN: 0267-8357; 1464-3804
• DOI: 10.1093/mutage/gex047

Anthracosilicosis (AS), a prevalent form of pneumoconiosis among coal miners, results from the accumulation of carbon and silica in the lungs from inhaled coal dust. This study investigated genotoxic effects and certain cytokine genes polymorphic variants in Russian coal miners with АS. Peripheral leukocytes were sampled from 129 patients with AS confirmed by X-ray and tissue biopsy and from 164 asymptomatic coal miners. Four single-nucleotide polymorphisms were genotyped in the extracted DNA samples: IL1β T-511C (rs16944), IL6 C-174G (rs1800795), IL12b A1188C (rs3212227) and VEGFA C634G (rs2010963). Genotoxic effects were assessed by the analysis of chromosome aberrations in cultured peripheral lymphocytes. The mean frequency of chromatid-type aberrations and chromosome-type aberrations, namely, chromatid-type breaks and dicentric chromosomes, was found to be higher in AS patients [3.70 (95% confidence interval {CI}, 3.29-4.10) and 0.28 (95% CI, 0.17-0.38)] compared to the control group [2.41 (95% CI, 2.00-2.82) and 0.09 (95% CI, 0.03-0.15)], respectively. IL1β gene T/T genotype (rs16944) was associated with AS [17.83% in AS patients against 4.35% in healthy donors, odds ratio = 4.77 (1.88-12.15), P < 0.01]. A significant increase in the level of certain chromosome interchanges among AS donors is of interest because such effects are typical for radiation damage and caused by acute oxidative stress. IL1β T allele probably may be considered as an AS susceptibility factor among coal miners.