Assertiveness of meat‐borne Lactococcus piscium strains and their potential for competitive exclusion of spoilage bacteria in situ and in vitro

Aims This study aimed to investigate intraspecies assertiveness of meat‐borne Lactococcus piscium isolates, inhibitory effects on unwanted and harmful meat spoilers, and the prevalence on beef deliberately inoculated with Lc. piscium. Methods and Results Co‐inoculation of Lc. piscium isolates and sp...

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Bibliographic Details
Published in:Journal of applied microbiology 2018-05, Vol.124 (5), p.1243-1253
Main Authors: Hilgarth, M., Nani, M., Vogel, R.F.
Format: Article
Language:English
Subjects:
assertiveness
Bacteria
Beef
bioprotective culture
competitive exclusion
Cultivation
Food safety
Hafnium oxide
Inoculation
Lactococcus piscium
Meat
meat spoilage
Microbiota
Product safety
Random amplified polymorphic DNA
Shelf life
Spoilage
Spoilers
Strains (organisms)
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Summary:Aims This study aimed to investigate intraspecies assertiveness of meat‐borne Lactococcus piscium isolates, inhibitory effects on unwanted and harmful meat spoilers, and the prevalence on beef deliberately inoculated with Lc. piscium. Methods and Results Co‐inoculation of Lc. piscium isolates and spoilers (Brochothrix thermosphacta, Leuconostoc gelidum subsp. gasicomitatum, Carnobacterium divergens, Pseudomonas weihenstephanensis, Serratia liquefaciens, Hafnia alvei) were conducted in sterile meat simulation medium. Differentiation of Lc. piscium strains was carried out with colony‐based RAPD‐PCR. Selective cultivation was used to differentiate spoilers from Lc. piscium. Intraspecies assertiveness revealed Lc. piscium TMW2.1614 as most assertive strain. Co‐inoculation of selected Lc. piscium strains caused substantial growth reduction of spoilers while the extent was strain‐ and spoiler dependent. Monitoring the microbiota on beef steaks deliberately inoculated with Lc. piscium revealed prevalence over the endogenous microbiota while maintaining a ripened sensory impression without undesired alterations. Conclusions This study reveals Lc. piscium strains TMW2.1612/2.1614/2.1615 as highly competitive against spoilers in vitro while beef deliberately inoculated with these strains maintained acceptable organoleptics. Significance and Impact of the Study Selected Lc. piscium strains exhibit high potential for application as bioprotective cultures for competitive exclusion on beef in order to extend minimum shelf life and enhance product safety of meat.
ISSN:1364-5072
1365-2672
DOI:10.1111/jam.13710