A fluorescent peptidyl substrate for visualizing peptidyl-prolyl cis/trans isomerase activity in live cells

This communication reports on a fluorescent probe (PPI-P) for imaging active peptidyl-prolyl cis/trans isomerases in live cells. PPI-P is capable of responding to both recombinant and cellular PPIases fluorogenically, and has been shown to specifically image active PPIases in live cells.

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2018, Vol.54 (15), p.1857-1860
Main Authors: Jiang, Quan, Li, Xiao-Rong, Wang, Cheng-Kun, Cheng, Juan, Tan, Chao, Cui, Tian-Tian, Lu, Nan-Nan, James, Tony D, Han, Feng, Li, Xin
Format: Article
Language:English
Subjects:
Cell Line
Cell Survival
Fluorescent Dyes - analysis
Fluorescent indicators
HEK293 Cells
Humans
Molecular Structure
Peptidylprolyl Isomerase - analysis
Peptidylprolyl Isomerase - metabolism
Spectrometry, Fluorescence
Substrate Specificity
Substrates
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Description
Summary:This communication reports on a fluorescent probe (PPI-P) for imaging active peptidyl-prolyl cis/trans isomerases in live cells. PPI-P is capable of responding to both recombinant and cellular PPIases fluorogenically, and has been shown to specifically image active PPIases in live cells.
ISSN:1359-7345
1364-548X
DOI:10.1039/c7cc09135d