Effect of systemic administration of lipopolysaccharides derived from Porphyromonas gingivalis on gene expression in mice kidney

• Published in: Medical molecular morphology 2018-09, Vol.51 (3), p.156-165
• Main Authors: Harada, Fumiya, Uehara, Osamu, Morikawa, Tetsuro, Hiraki, Daichi, Onishi, Aya, Toraya, Seiko, Adhikari, Bhoj Raj, Takai, Rie, Yoshida, Koki, Sato, Jun, Nishimura, Michiko, Chiba, Itsuo, Wu, Ching Zong, Abiko, Yoshihiro
• Format: Article
• Language: English
• Subjects: Anatomy; Animals; Cells, Cultured; Cytokines - immunology; Cytokines - metabolism; Disease Models, Animal; DNA microarrays; Endothelial cells; Endothelial Cells - immunology; Endothelial Cells - metabolism; Gene expression; Gene Expression Profiling; Gum disease; Humans; Kidney - immunology; Kidney - pathology; Kidney diseases; Lipopolysaccharides; Lipopolysaccharides - immunology; Male; Medicine; Medicine & Public Health; Mice; Mice, Inbred C57BL; Molecular Medicine; Oligonucleotide Array Sequence Analysis; Original Paper; Pathology; Periodontitis; Periodontitis - complications; Periodontitis - microbiology; Periodontitis - pathology; Polymerase chain reaction; Porphyromonas gingivalis; Porphyromonas gingivalis - metabolism; Primary Cell Culture; Renal Insufficiency, Chronic - etiology; Renal Insufficiency, Chronic - pathology; RNA, Messenger - metabolism; Rodents; Up-Regulation
• ISSN: 1860-1480; 1860-1499
• DOI: 10.1007/s00795-018-0181-3

Although an association between periodontitis and chronic kidney disease (CKD) has been suggested, the mechanism involved remains unclear. Herein, we examined the global gene expression profile in a mouse model that showed no acute inflammation in the kidney following stimulation with lipopolysaccharides (LPS) derived from Porphyromonas gingivalis (PG-LPS). The mice were injected with PG-LPS at a concentration of 5 mg/kg intraperitoneally, every 3 days, for 1 month. Microarray analysis was used to identify 10 genes with the highest expression levels in the kidney stimulated with PG-LPS. Among them, the functions of five genes ( Saa3, Ticam2, Reg3b, Ocxt2a , and Xcr1 ) were known. The upregulation of these genes was confirmed by quantitative polymerase chain reaction assay. Furthermore, we examined whether the expression of these upregulated genes were altered in endothelial cells derived from the kidney, in vitro. The mRNA expression levels of all five genes were significantly higher in the experimental group than in the controls (no LPS stimulation; * p