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Effect of systemic administration of lipopolysaccharides derived from Porphyromonas gingivalis on gene expression in mice kidney
Although an association between periodontitis and chronic kidney disease (CKD) has been suggested, the mechanism involved remains unclear. Herein, we examined the global gene expression profile in a mouse model that showed no acute inflammation in the kidney following stimulation with lipopolysaccha...
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| Published in: | Medical molecular morphology 2018-09, Vol.51 (3), p.156-165 |
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| creator | Harada, Fumiya Uehara, Osamu Morikawa, Tetsuro Hiraki, Daichi Onishi, Aya Toraya, Seiko Adhikari, Bhoj Raj Takai, Rie Yoshida, Koki Sato, Jun Nishimura, Michiko Chiba, Itsuo Wu, Ching Zong Abiko, Yoshihiro |
| description | Although an association between periodontitis and chronic kidney disease (CKD) has been suggested, the mechanism involved remains unclear. Herein, we examined the global gene expression profile in a mouse model that showed no acute inflammation in the kidney following stimulation with lipopolysaccharides (LPS) derived from
Porphyromonas gingivalis
(PG-LPS). The mice were injected with PG-LPS at a concentration of 5 mg/kg intraperitoneally, every 3 days, for 1 month. Microarray analysis was used to identify 10 genes with the highest expression levels in the kidney stimulated with PG-LPS. Among them, the functions of five genes (
Saa3, Ticam2, Reg3b, Ocxt2a
, and
Xcr1
) were known. The upregulation of these genes was confirmed by quantitative polymerase chain reaction assay. Furthermore, we examined whether the expression of these upregulated genes were altered in endothelial cells derived from the kidney, in vitro. The mRNA expression levels of all five genes were significantly higher in the experimental group than in the controls (no LPS stimulation; *
p
|
| doi_str_mv | 10.1007/s00795-018-0181-3 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1993387211</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1993603790</sourcerecordid><originalsourceid>FETCH-LOGICAL-c462t-3e8d60fe6520fb3d55114d9052059f3c85188802927fcd67b2d6740ed94434e03</originalsourceid><addsrcrecordid>eNp1Uctu1TAQtRCIPuAD2CBLbLoJ-JGHvayq0laqBAtYR772-NYlsVNPbkV2fDpOb6mqSixmPNacc2bsQ8gHzj5zxrovWJJuKsbVGrySr8ghVy2reK3166dasQNyhHjLmOxa0bwlB0JLpVijDsmfc-_BzjR5igvOMAZLjRtDDDhnM4cU19YQpjSlYUFj7Y3JwQFSBzncg6M-p5F-T3m6WUqVokG6DXEb7s0QkBb-FiJQ-D1lQFz1QqRlCtBfwUVY3pE33gwI7x_PY_Lz6_mPs8vq-tvF1dnpdWXrVsyVBOVa5qFtBPMb6ZqG89ppVq6N9tKqhqvyJKFF561ru40oqWbgdF3LGpg8Jid73Smnux3g3I8BLQyDiZB22HOtpVSd4LxAP72A3qZdjmW7B1RbvlGvgnyPsjkhZvD9lMNo8tJz1q_29Ht7-mLNGryXhfPxUXm3GcE9Mf75UQBiD8DSilvIz0b_V_Uvevab_Q</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1993603790</pqid></control><display><type>article</type><title>Effect of systemic administration of lipopolysaccharides derived from Porphyromonas gingivalis on gene expression in mice kidney</title><source>Springer Nature</source><creator>Harada, Fumiya ; Uehara, Osamu ; Morikawa, Tetsuro ; Hiraki, Daichi ; Onishi, Aya ; Toraya, Seiko ; Adhikari, Bhoj Raj ; Takai, Rie ; Yoshida, Koki ; Sato, Jun ; Nishimura, Michiko ; Chiba, Itsuo ; Wu, Ching Zong ; Abiko, Yoshihiro</creator><creatorcontrib>Harada, Fumiya ; Uehara, Osamu ; Morikawa, Tetsuro ; Hiraki, Daichi ; Onishi, Aya ; Toraya, Seiko ; Adhikari, Bhoj Raj ; Takai, Rie ; Yoshida, Koki ; Sato, Jun ; Nishimura, Michiko ; Chiba, Itsuo ; Wu, Ching Zong ; Abiko, Yoshihiro</creatorcontrib><description>Although an association between periodontitis and chronic kidney disease (CKD) has been suggested, the mechanism involved remains unclear. Herein, we examined the global gene expression profile in a mouse model that showed no acute inflammation in the kidney following stimulation with lipopolysaccharides (LPS) derived from
Porphyromonas gingivalis
(PG-LPS). The mice were injected with PG-LPS at a concentration of 5 mg/kg intraperitoneally, every 3 days, for 1 month. Microarray analysis was used to identify 10 genes with the highest expression levels in the kidney stimulated with PG-LPS. Among them, the functions of five genes (
Saa3, Ticam2, Reg3b, Ocxt2a
, and
Xcr1
) were known. The upregulation of these genes was confirmed by quantitative polymerase chain reaction assay. Furthermore, we examined whether the expression of these upregulated genes were altered in endothelial cells derived from the kidney, in vitro. The mRNA expression levels of all five genes were significantly higher in the experimental group than in the controls (no LPS stimulation; *
p
< 0.05). In conclusion, the responses noted in the kidney may have arisen mainly from the endothelial cells. Moreover, upregulation of the expression levels of
Saa3, Ticam2, Reg3b, Ocxt2a
, and
Xcr1
may be associated with the pathogenesis of CKD.</description><identifier>ISSN: 1860-1480</identifier><identifier>EISSN: 1860-1499</identifier><identifier>DOI: 10.1007/s00795-018-0181-3</identifier><identifier>PMID: 29388058</identifier><language>eng</language><publisher>Tokyo: Springer Japan</publisher><subject>Anatomy ; DNA microarrays ; Endothelial cells ; Gene expression ; Gum disease ; Kidney diseases ; Lipopolysaccharides ; Medicine ; Medicine & Public Health ; Molecular Medicine ; Original Paper ; Pathology ; Periodontitis ; Polymerase chain reaction ; Porphyromonas gingivalis ; Rodents</subject><ispartof>Medical molecular morphology, 2018-09, Vol.51 (3), p.156-165</ispartof><rights>The Japanese Society for Clinical Molecular Morphology 2018</rights><rights>Medical Molecular Morphology is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c462t-3e8d60fe6520fb3d55114d9052059f3c85188802927fcd67b2d6740ed94434e03</citedby><cites>FETCH-LOGICAL-c462t-3e8d60fe6520fb3d55114d9052059f3c85188802927fcd67b2d6740ed94434e03</cites><orcidid>0000-0002-8312-5903</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29388058$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Harada, Fumiya</creatorcontrib><creatorcontrib>Uehara, Osamu</creatorcontrib><creatorcontrib>Morikawa, Tetsuro</creatorcontrib><creatorcontrib>Hiraki, Daichi</creatorcontrib><creatorcontrib>Onishi, Aya</creatorcontrib><creatorcontrib>Toraya, Seiko</creatorcontrib><creatorcontrib>Adhikari, Bhoj Raj</creatorcontrib><creatorcontrib>Takai, Rie</creatorcontrib><creatorcontrib>Yoshida, Koki</creatorcontrib><creatorcontrib>Sato, Jun</creatorcontrib><creatorcontrib>Nishimura, Michiko</creatorcontrib><creatorcontrib>Chiba, Itsuo</creatorcontrib><creatorcontrib>Wu, Ching Zong</creatorcontrib><creatorcontrib>Abiko, Yoshihiro</creatorcontrib><title>Effect of systemic administration of lipopolysaccharides derived from Porphyromonas gingivalis on gene expression in mice kidney</title><title>Medical molecular morphology</title><addtitle>Med Mol Morphol</addtitle><addtitle>Med Mol Morphol</addtitle><description>Although an association between periodontitis and chronic kidney disease (CKD) has been suggested, the mechanism involved remains unclear. Herein, we examined the global gene expression profile in a mouse model that showed no acute inflammation in the kidney following stimulation with lipopolysaccharides (LPS) derived from
Porphyromonas gingivalis
(PG-LPS). The mice were injected with PG-LPS at a concentration of 5 mg/kg intraperitoneally, every 3 days, for 1 month. Microarray analysis was used to identify 10 genes with the highest expression levels in the kidney stimulated with PG-LPS. Among them, the functions of five genes (
Saa3, Ticam2, Reg3b, Ocxt2a
, and
Xcr1
) were known. The upregulation of these genes was confirmed by quantitative polymerase chain reaction assay. Furthermore, we examined whether the expression of these upregulated genes were altered in endothelial cells derived from the kidney, in vitro. The mRNA expression levels of all five genes were significantly higher in the experimental group than in the controls (no LPS stimulation; *
p
< 0.05). In conclusion, the responses noted in the kidney may have arisen mainly from the endothelial cells. Moreover, upregulation of the expression levels of
Saa3, Ticam2, Reg3b, Ocxt2a
, and
Xcr1
may be associated with the pathogenesis of CKD.</description><subject>Anatomy</subject><subject>DNA microarrays</subject><subject>Endothelial cells</subject><subject>Gene expression</subject><subject>Gum disease</subject><subject>Kidney diseases</subject><subject>Lipopolysaccharides</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Molecular Medicine</subject><subject>Original Paper</subject><subject>Pathology</subject><subject>Periodontitis</subject><subject>Polymerase chain reaction</subject><subject>Porphyromonas gingivalis</subject><subject>Rodents</subject><issn>1860-1480</issn><issn>1860-1499</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp1Uctu1TAQtRCIPuAD2CBLbLoJ-JGHvayq0laqBAtYR772-NYlsVNPbkV2fDpOb6mqSixmPNacc2bsQ8gHzj5zxrovWJJuKsbVGrySr8ghVy2reK3166dasQNyhHjLmOxa0bwlB0JLpVijDsmfc-_BzjR5igvOMAZLjRtDDDhnM4cU19YQpjSlYUFj7Y3JwQFSBzncg6M-p5F-T3m6WUqVokG6DXEb7s0QkBb-FiJQ-D1lQFz1QqRlCtBfwUVY3pE33gwI7x_PY_Lz6_mPs8vq-tvF1dnpdWXrVsyVBOVa5qFtBPMb6ZqG89ppVq6N9tKqhqvyJKFF561ru40oqWbgdF3LGpg8Jid73Smnux3g3I8BLQyDiZB22HOtpVSd4LxAP72A3qZdjmW7B1RbvlGvgnyPsjkhZvD9lMNo8tJz1q_29Ht7-mLNGryXhfPxUXm3GcE9Mf75UQBiD8DSilvIz0b_V_Uvevab_Q</recordid><startdate>20180901</startdate><enddate>20180901</enddate><creator>Harada, Fumiya</creator><creator>Uehara, Osamu</creator><creator>Morikawa, Tetsuro</creator><creator>Hiraki, Daichi</creator><creator>Onishi, Aya</creator><creator>Toraya, Seiko</creator><creator>Adhikari, Bhoj Raj</creator><creator>Takai, Rie</creator><creator>Yoshida, Koki</creator><creator>Sato, Jun</creator><creator>Nishimura, Michiko</creator><creator>Chiba, Itsuo</creator><creator>Wu, Ching Zong</creator><creator>Abiko, Yoshihiro</creator><general>Springer Japan</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-8312-5903</orcidid></search><sort><creationdate>20180901</creationdate><title>Effect of systemic administration of lipopolysaccharides derived from Porphyromonas gingivalis on gene expression in mice kidney</title><author>Harada, Fumiya ; Uehara, Osamu ; Morikawa, Tetsuro ; Hiraki, Daichi ; Onishi, Aya ; Toraya, Seiko ; Adhikari, Bhoj Raj ; Takai, Rie ; Yoshida, Koki ; Sato, Jun ; Nishimura, Michiko ; Chiba, Itsuo ; Wu, Ching Zong ; Abiko, Yoshihiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c462t-3e8d60fe6520fb3d55114d9052059f3c85188802927fcd67b2d6740ed94434e03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Anatomy</topic><topic>DNA microarrays</topic><topic>Endothelial cells</topic><topic>Gene expression</topic><topic>Gum disease</topic><topic>Kidney diseases</topic><topic>Lipopolysaccharides</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Molecular Medicine</topic><topic>Original Paper</topic><topic>Pathology</topic><topic>Periodontitis</topic><topic>Polymerase chain reaction</topic><topic>Porphyromonas gingivalis</topic><topic>Rodents</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Harada, Fumiya</creatorcontrib><creatorcontrib>Uehara, Osamu</creatorcontrib><creatorcontrib>Morikawa, Tetsuro</creatorcontrib><creatorcontrib>Hiraki, Daichi</creatorcontrib><creatorcontrib>Onishi, Aya</creatorcontrib><creatorcontrib>Toraya, Seiko</creatorcontrib><creatorcontrib>Adhikari, Bhoj Raj</creatorcontrib><creatorcontrib>Takai, Rie</creatorcontrib><creatorcontrib>Yoshida, Koki</creatorcontrib><creatorcontrib>Sato, Jun</creatorcontrib><creatorcontrib>Nishimura, Michiko</creatorcontrib><creatorcontrib>Chiba, Itsuo</creatorcontrib><creatorcontrib>Wu, Ching Zong</creatorcontrib><creatorcontrib>Abiko, Yoshihiro</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Proquest Nursing & Allied Health Source</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><jtitle>Medical molecular morphology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Harada, Fumiya</au><au>Uehara, Osamu</au><au>Morikawa, Tetsuro</au><au>Hiraki, Daichi</au><au>Onishi, Aya</au><au>Toraya, Seiko</au><au>Adhikari, Bhoj Raj</au><au>Takai, Rie</au><au>Yoshida, Koki</au><au>Sato, Jun</au><au>Nishimura, Michiko</au><au>Chiba, Itsuo</au><au>Wu, Ching Zong</au><au>Abiko, Yoshihiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of systemic administration of lipopolysaccharides derived from Porphyromonas gingivalis on gene expression in mice kidney</atitle><jtitle>Medical molecular morphology</jtitle><stitle>Med Mol Morphol</stitle><addtitle>Med Mol Morphol</addtitle><date>2018-09-01</date><risdate>2018</risdate><volume>51</volume><issue>3</issue><spage>156</spage><epage>165</epage><pages>156-165</pages><issn>1860-1480</issn><eissn>1860-1499</eissn><abstract>Although an association between periodontitis and chronic kidney disease (CKD) has been suggested, the mechanism involved remains unclear. Herein, we examined the global gene expression profile in a mouse model that showed no acute inflammation in the kidney following stimulation with lipopolysaccharides (LPS) derived from
Porphyromonas gingivalis
(PG-LPS). The mice were injected with PG-LPS at a concentration of 5 mg/kg intraperitoneally, every 3 days, for 1 month. Microarray analysis was used to identify 10 genes with the highest expression levels in the kidney stimulated with PG-LPS. Among them, the functions of five genes (
Saa3, Ticam2, Reg3b, Ocxt2a
, and
Xcr1
) were known. The upregulation of these genes was confirmed by quantitative polymerase chain reaction assay. Furthermore, we examined whether the expression of these upregulated genes were altered in endothelial cells derived from the kidney, in vitro. The mRNA expression levels of all five genes were significantly higher in the experimental group than in the controls (no LPS stimulation; *
p
< 0.05). In conclusion, the responses noted in the kidney may have arisen mainly from the endothelial cells. Moreover, upregulation of the expression levels of
Saa3, Ticam2, Reg3b, Ocxt2a
, and
Xcr1
may be associated with the pathogenesis of CKD.</abstract><cop>Tokyo</cop><pub>Springer Japan</pub><pmid>29388058</pmid><doi>10.1007/s00795-018-0181-3</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-8312-5903</orcidid></addata></record> |
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| source | Springer Nature |
| subjects | Anatomy DNA microarrays Endothelial cells Gene expression Gum disease Kidney diseases Lipopolysaccharides Medicine Medicine & Public Health Molecular Medicine Original Paper Pathology Periodontitis Polymerase chain reaction Porphyromonas gingivalis Rodents |
| title | Effect of systemic administration of lipopolysaccharides derived from Porphyromonas gingivalis on gene expression in mice kidney |
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