Mass spectrometric sequencing of endotoxin proteins of Bacillus thuringiensis ssp. konkukian extracted from polyacrylamide gels

• Published in: Proteomics (Weinheim) 2006-03, Vol.6 (5), p.1512-1517
• Main Authors: Lee, Kwang Yong, Kang, Eun Young, Park, Sooil, Ahn, Seong Kyu, Yoo, Kwan Hee, Kim, Jin Young, Lee, Hyung Hoan
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Analytical, structural and metabolic biochemistry; Bacillus thuringiensis; Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Bacterial Toxins - chemistry; Bacterial Toxins - genetics; Biological and medical sciences; Electrophoresis, Polyacrylamide Gel; Endotoxin crystal; Endotoxins - chemistry; Endotoxins - genetics; ESI-Q-TOF mass spectrometry; Fundamental and applied biological sciences. Psychology; Hemolysin Proteins; Insecticides - chemistry; Mass Spectrometry; Miscellaneous; Molecular Sequence Data; Peptides - chemistry; Peptides - genetics; Proteins
• ISSN: 1615-9853; 1615-9861
• DOI: 10.1002/pmic.200500298

The amino acid sequences of the crystal proteins of Bacillus thuringiensis ssp. konkukian strain HL‐47 are unknown. We used 1‐D denaturing polyacrylamide electrophoresis, nano‐ESI‐Q‐TOF‐MS, and protein database searching to analyze these proteins. On SDS‐PAGE gels, a preparation of purified crystal proteins exhibited 110, 102, 76, 55, 37, and 30 kDa protein bands. Immunoblotting of the gel with antiserum raised to this preparation revealed that four crystal proteins, of 110, 102, 55, and 37 kDa, reacted with the specific antiserum. The 102‐kDa major protein reacted strongly. The other crystal proteins showed weak immunoreactivity. The 102 and 55 kDa proteins were analyzed by ESI‐MS. The internal amino acid sequence of the 102‐kDa major protein has similarity to the sequences of the surface layer protein of B. thuringiensis ssp. finitimus and B. anthracis. However, the internal amino acid sequences of the 55 kDa protein did not show any homology to proteins in the databases. Proteomic analysis of these proteins leads to the conclusion that the sequence data provided the protein databases of the crystal proteins of the konkukian ssp.