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RNA interference technology to improve the baculovirus-insect cell expression system
The baculovirus expression vector system (BEVS) is a popular manufacturing platform for the production of recombinant proteins, antiviral vaccines, gene therapy vectors, and biopesticides. Besides its successful applications in the industrial sector, the system has also played a significant role wit...
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Published in: | Biotechnology advances 2018-03, Vol.36 (2), p.443-451 |
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description | The baculovirus expression vector system (BEVS) is a popular manufacturing platform for the production of recombinant proteins, antiviral vaccines, gene therapy vectors, and biopesticides. Besides its successful applications in the industrial sector, the system has also played a significant role within the academic community given its extensive use in the production of hard-to-express eukaryotic multiprotein complexes for structural characterization for example. However, as other expression platforms, BEVS has to be continually improved to overcome its limitation and adapt to the constant demand for manufacturing processes that provide recombinant products with improved quality at higher yields and lower production cost.
RNA interference, or RNAi, is a relatively recent technology that has revolutionized how scientist study gene function. Originally introduced as a tool to study biological and disease-related processes it has recently been applied to improve the yield and quality of recombinant proteins produced in several expression systems. In this review, we provide a comprehensive summary of the impact that RNAi-mediated silencing of cellular or viral genes in the BEVS has on the production of recombinant products. We also propose a critical analysis of several aspects of the methodologies described in the literature for the use of RNAi technology in the BEVS with the intent to provide the reader with eventually useful guidance for designing experiments. |
doi_str_mv | 10.1016/j.biotechadv.2018.01.008 |
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RNA interference, or RNAi, is a relatively recent technology that has revolutionized how scientist study gene function. Originally introduced as a tool to study biological and disease-related processes it has recently been applied to improve the yield and quality of recombinant proteins produced in several expression systems. In this review, we provide a comprehensive summary of the impact that RNAi-mediated silencing of cellular or viral genes in the BEVS has on the production of recombinant products. We also propose a critical analysis of several aspects of the methodologies described in the literature for the use of RNAi technology in the BEVS with the intent to provide the reader with eventually useful guidance for designing experiments.</description><identifier>ISSN: 0734-9750</identifier><identifier>EISSN: 1873-1899</identifier><identifier>DOI: 10.1016/j.biotechadv.2018.01.008</identifier><identifier>PMID: 29395442</identifier><language>eng</language><publisher>England: Elsevier Inc</publisher><subject>Animals ; Baculoviridae - genetics ; Baculoviridae - metabolism ; Baculovirus ; Baculovirus expression system ; Biological activity ; Bioreactors ; Bombyx ; Cell Line ; Cellular manufacture ; Gene expression ; Gene therapy ; Interference ; Metabolic Engineering ; Pesticides ; Proteins ; Recombinant protein ; Recombinant Proteins - genetics ; Recombinant Proteins - metabolism ; Ribonucleic acid ; RNA ; RNA Interference ; Sf9 Cells ; siRNA ; Structural analysis ; Vaccines ; Vaccines manufacturing</subject><ispartof>Biotechnology advances, 2018-03, Vol.36 (2), p.443-451</ispartof><rights>2018 Elsevier Inc.</rights><rights>Copyright © 2018 Elsevier Inc. All rights reserved.</rights><rights>Copyright Elsevier Science Ltd. Mar/Apr 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c505t-19092f05d09c2e35660460f358a2a4928c46cdb7b2940df3f5c59e1afb7e85963</citedby><cites>FETCH-LOGICAL-c505t-19092f05d09c2e35660460f358a2a4928c46cdb7b2940df3f5c59e1afb7e85963</cites><orcidid>0000-0001-9110-8815</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29395442$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chavez-Pena, Cuitlahuac</creatorcontrib><creatorcontrib>Kamen, Amine A.</creatorcontrib><title>RNA interference technology to improve the baculovirus-insect cell expression system</title><title>Biotechnology advances</title><addtitle>Biotechnol Adv</addtitle><description>The baculovirus expression vector system (BEVS) is a popular manufacturing platform for the production of recombinant proteins, antiviral vaccines, gene therapy vectors, and biopesticides. Besides its successful applications in the industrial sector, the system has also played a significant role within the academic community given its extensive use in the production of hard-to-express eukaryotic multiprotein complexes for structural characterization for example. However, as other expression platforms, BEVS has to be continually improved to overcome its limitation and adapt to the constant demand for manufacturing processes that provide recombinant products with improved quality at higher yields and lower production cost.
RNA interference, or RNAi, is a relatively recent technology that has revolutionized how scientist study gene function. Originally introduced as a tool to study biological and disease-related processes it has recently been applied to improve the yield and quality of recombinant proteins produced in several expression systems. In this review, we provide a comprehensive summary of the impact that RNAi-mediated silencing of cellular or viral genes in the BEVS has on the production of recombinant products. We also propose a critical analysis of several aspects of the methodologies described in the literature for the use of RNAi technology in the BEVS with the intent to provide the reader with eventually useful guidance for designing experiments.</description><subject>Animals</subject><subject>Baculoviridae - genetics</subject><subject>Baculoviridae - metabolism</subject><subject>Baculovirus</subject><subject>Baculovirus expression system</subject><subject>Biological activity</subject><subject>Bioreactors</subject><subject>Bombyx</subject><subject>Cell Line</subject><subject>Cellular manufacture</subject><subject>Gene expression</subject><subject>Gene therapy</subject><subject>Interference</subject><subject>Metabolic Engineering</subject><subject>Pesticides</subject><subject>Proteins</subject><subject>Recombinant protein</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - metabolism</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA Interference</subject><subject>Sf9 Cells</subject><subject>siRNA</subject><subject>Structural analysis</subject><subject>Vaccines</subject><subject>Vaccines manufacturing</subject><issn>0734-9750</issn><issn>1873-1899</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNqFkE1r3DAQhkVJ6G7T_oUi6CUXu6Mv2zqmoUkDIYGQnIUtj7tabGsj2Uv231dm0wZ6yUkgnpn3nYcQyiBnwIrv27xxfkK7qdt9zoFVObAcoPpA1qwqRcYqrU_IGkohM10qWJFPMW4BmAIlPpIV10IrKfmaPD7cXVA3Thg6DDhapMva0ff-94FOnrphF_w-_W6QNrWde793YY6ZGyPaiVrse4ovu4AxOj_SeIgTDp_JaVf3Eb-8vmfk6ern4-Wv7Pb--uby4jazqceUMQ2ad6Ba0JajUEUBsoBOqKrmtdS8srKwbVM2XEtoO9EpqzSyumtKrJQuxBk5P-5NHZ9njJMZXFwq1SP6ORqm051ailIk9Nt_6NbPYUztDIdClClYlomqjpQNPsaAndkFN9ThYBiYxbzZmjfzZjFvgJlkPo1-fQ2YmwHbf4N_VSfgxxHAZGTvMJho3WK8dSGpNK1376f8AZjembs</recordid><startdate>20180301</startdate><enddate>20180301</enddate><creator>Chavez-Pena, Cuitlahuac</creator><creator>Kamen, Amine A.</creator><general>Elsevier Inc</general><general>Elsevier Science Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QQ</scope><scope>7SE</scope><scope>7TA</scope><scope>7TB</scope><scope>8FD</scope><scope>FR3</scope><scope>H8G</scope><scope>JG9</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-9110-8815</orcidid></search><sort><creationdate>20180301</creationdate><title>RNA interference technology to improve the baculovirus-insect cell expression system</title><author>Chavez-Pena, Cuitlahuac ; Kamen, Amine A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c505t-19092f05d09c2e35660460f358a2a4928c46cdb7b2940df3f5c59e1afb7e85963</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Baculoviridae - genetics</topic><topic>Baculoviridae - metabolism</topic><topic>Baculovirus</topic><topic>Baculovirus expression system</topic><topic>Biological activity</topic><topic>Bioreactors</topic><topic>Bombyx</topic><topic>Cell Line</topic><topic>Cellular manufacture</topic><topic>Gene expression</topic><topic>Gene therapy</topic><topic>Interference</topic><topic>Metabolic Engineering</topic><topic>Pesticides</topic><topic>Proteins</topic><topic>Recombinant protein</topic><topic>Recombinant Proteins - genetics</topic><topic>Recombinant Proteins - metabolism</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA Interference</topic><topic>Sf9 Cells</topic><topic>siRNA</topic><topic>Structural analysis</topic><topic>Vaccines</topic><topic>Vaccines manufacturing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chavez-Pena, Cuitlahuac</creatorcontrib><creatorcontrib>Kamen, Amine A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Ceramic Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>MEDLINE - Academic</collection><jtitle>Biotechnology advances</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chavez-Pena, Cuitlahuac</au><au>Kamen, Amine A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>RNA interference technology to improve the baculovirus-insect cell expression system</atitle><jtitle>Biotechnology advances</jtitle><addtitle>Biotechnol Adv</addtitle><date>2018-03-01</date><risdate>2018</risdate><volume>36</volume><issue>2</issue><spage>443</spage><epage>451</epage><pages>443-451</pages><issn>0734-9750</issn><eissn>1873-1899</eissn><abstract>The baculovirus expression vector system (BEVS) is a popular manufacturing platform for the production of recombinant proteins, antiviral vaccines, gene therapy vectors, and biopesticides. 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RNA interference, or RNAi, is a relatively recent technology that has revolutionized how scientist study gene function. Originally introduced as a tool to study biological and disease-related processes it has recently been applied to improve the yield and quality of recombinant proteins produced in several expression systems. In this review, we provide a comprehensive summary of the impact that RNAi-mediated silencing of cellular or viral genes in the BEVS has on the production of recombinant products. We also propose a critical analysis of several aspects of the methodologies described in the literature for the use of RNAi technology in the BEVS with the intent to provide the reader with eventually useful guidance for designing experiments.</abstract><cop>England</cop><pub>Elsevier Inc</pub><pmid>29395442</pmid><doi>10.1016/j.biotechadv.2018.01.008</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-9110-8815</orcidid></addata></record> |
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subjects | Animals Baculoviridae - genetics Baculoviridae - metabolism Baculovirus Baculovirus expression system Biological activity Bioreactors Bombyx Cell Line Cellular manufacture Gene expression Gene therapy Interference Metabolic Engineering Pesticides Proteins Recombinant protein Recombinant Proteins - genetics Recombinant Proteins - metabolism Ribonucleic acid RNA RNA Interference Sf9 Cells siRNA Structural analysis Vaccines Vaccines manufacturing |
title | RNA interference technology to improve the baculovirus-insect cell expression system |
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